Course Director: Laurence Morel, Ph.D. E-mail: morel@pathology.ufl.edu
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Date |
Topic |
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1 |
August 24 |
Lecture: Mouse Genomic facts,
strains, crosses, |
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2 |
August 26 |
Lecture: Mapping genes |
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3
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August 29 |
Paper discussion: Mapping the Clock
gene (I) |
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4
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August 31 |
Paper discussion: Mapping the Clock
gene (II) |
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5
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September 2 |
Paper discussion: Complex trait
mapping |
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6 |
September 7 |
Paper
discussion: Congenics |
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7
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September 9 |
Paper discussion: The mouse genome
project |
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8
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September 12 |
Paper discussion: Comparative
genomics Database demonstration |
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9
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September 14 |
Paper discussion: Inducible
transgenes |
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10 |
September 16 |
Paper discussion: Inducible
tissue-specific knock-outs |
|
11 |
September 19 |
Paper discussion: Genetics
modifiers |
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12 |
September 21 |
Paper discussion: ENU mutagenesis |
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13 |
September 23 |
Lecture: Transgenics / Knock outs |
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14 |
September 26 |
Paper discussion: imprinting |
Recommended
textbook: Lecture:
Mouse Genetics: concepts and applications, by Lee M. Silver, 1995, Oxford University Press
The book is out of print. The text is on-line at http://www.informatics.jax.org/silver/
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Class |
Required reading |
|
1 |
Silver: Chapters 1, 3.1, 3.2, 3.4,
5.1. |
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2 |
Silver: Chapters 7, 8.3.6, 9.3 –
9.5 |
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3
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Review: Takahashi
J.S. et al. Forward and reverse genetic approaches to behavior in the mouse.
Science, 264: 1724-33 (1994). Vitaterna
M.H. et al. Mutagenesis and
mapping of a mouse gene, Clock,
essential for circadian behavior.
Science 264: 719-725 (1994) |
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4
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King D.P. et al. Positional cloning of the mouse circadian Clock gene. Cell, 89:
641-653 (1997). Antoch M.P.,
et al. Functional identification
of the mouse circadian Clock
gene by transgenic BAC rescue. Cell,
89: 655-667 (1997). |
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5
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Review: Doerge
R.W. Mapping
and analysis of quantitative trait loci in experimental populations.
Nature Rev. Genetics 3: 43-52
(2002).
online
Glazier
A.M., Nadeau J.H., Aitman T.J. Finding
genes that underlie complex traits. Science, 298: 2345-2349 (2002).
online1
online2 Todd
J.A., et al. Genetic analysis of
autoimmune type 1 diabetes mellitus in mice. Nature, 351: 542-6
(1991). Wicker L.S., et al. Fine mapping, gene content, comparative sequencing, and expression
analyses support Ctla4 and Nramp1 as candidates for Idd5.1 and Idd5.2 in
the nonobese diabetic mouse. online
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6
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Review: Rogner U.C and Avner P. Congenic mice: Cutting tools for complex immune disorders. Nature Rev. Genetics, 3: 243-251(2003). online Wakeland
E.K. et al. Speed congenics:
A classic technique moves
into the fast lane (relatively speaking). Immunol.
Today, 18: 473-7 (1997).
online Markel P. et al. Theoritical
and empirical issues for marker-assisted breeding of congenic mouse
strains. Nat. Genetics, 17: 280-84 (1997) |
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7
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Review: Green
E.D. at al. Strategies for the
systematic sequencing of complex genomes.
Nature Rev. Genetics, 2:
573-583 (2001).
online
Mouse
Genome Sequencing consortium. Initial sequencing and comparative analysis of the mouse genome. Nature,
420: 520-562 (2002). online
The
FANTOM Consortium and the Riken Genome Exploration Research Group Phase
I & II team. Analysis
of the mouse transcriptome based on functional annotation of 60,770
full-length cDNAs. Nature,
420: 563-573 (2002). online
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8
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Review: Nadeau J.H., Sankoff D: Counting on comparative maps. Trends Genet., 14:495-501 (1998). online Ureta-Vidal A. et al. Comparative genomics: Genome-wide
analysis in metazoan eukaryotes. Nature
Rev. Genetics, 4: 251-262
(2003).
online
Gregory,
S. G., et al. A physical map of
the mouse genome. Nature, 418:743-750
(2002). online Thomas
J.W. et al. Comparative analyses
of multi-species sequences from targeted genomic regions.
Nature, 424:
788-793 (2003). online
Hand-on session: using mouse genomic databases Varmus
H. Genomic empowerment: The
importance of public database. Nat.
Genetics Supplement, p. 3, (Sept. 2002).
online
Question 1: How does one find a gene of interest and determine that gene's structure? Once the gene has been located on the map, how does one easily examine other genes in that same region? online pp 9 – 17. Question
7: How would an investigator easily find compiled information describing
the structure of a gene of interest? Is it possible to obtain the
sequence of any putative promoter regions?
online
Question 12: How does a user find characterized mouse mutants corresponding to human genes? online Question 13: A user has
identified an interesting phenotype in a mouse model and has been able
to narrow down the critical region for the responsible gene to
approximately 0.5 cM. How does one find the mouse genes in this region?
online
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9
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Review: Lewandoski, M. Conditional
control of gene expression in the mouse. Nature Rev. Genetics, 2:
743-755 (2001).
online Shin,
M.K. et al. The temporal
requirement for endothelin receptor-B signaling during neural crest
development. Nature, 402: 496-501 (1999). online
Wang
X. L. et al. Development of
gene-switch transgenic mice that inducibly express TGFbeta1 in the
epidermis. Proc. Natl. Acad.
Sci., 96: 8483-8488
(1999). online
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|
10 |
Meyers,
E. N. et al. An Fgf8 mutant
allelic series generated by Cre- and Flp-mediated recombination. Nat.
Genetics, 18: 136-141 (1998). Tannour-Louet,
M. et al. A tamoxifen-inducible chimeric Cre recombinase especifically effective
in the fetal and adult mouse liver.
Hepatology, 35:
1072-1081 (2002). |
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11 |
Review: Nadeau, J.H. Modifier genes in mice and humans. Nature Rev. Genetics, 2: 165- 174 (2001). online Bonyadi
M. et al. Mapping of a major
genetic modifier of embryonic lethality in TGF beta 1 knockout mice. Nat.
Genetics, 15: 207-11 (1997) Gould K.A., et al. Genetic
evaluation of candidate genes for the Mom1
modifier of intestinal neoplasia in mice. Genetics,
144:1777-85 (1996) |
|
12 |
Nelms, K.A., and Goodnow, C.C.
Genome-wide ENU mutagenesis to reveal immune regulators. Immunity,
15: 409-18 (2001). online
Papathanasiou, P. et al. Widespread Failure of Hematolymphoid Differentiation Caused by a Recessive Niche-Filling Allele of the Ikaros Transcription Factor. Immunity, 19: 131-144 (2003). online Du,
X. et al. Velvet, a dominant Egfr
mutation that causes wavy hair and defective eyelid development in mice.
Genetics, 166: 331-340 (2004). online |
|
13 |
Dr. Scott’s lecture on transgenics |
|
14 |
Review: Wilkins J.F. and Haig, D. What good is genomic imprinting: the function of parent-specific gene expression. Nature Rev. Genetics, 4: 1-10 (2003). online Verona,
R.I. et al. Genomic imprinting:
Intricacies of epigenetic regulation in clusters.
Annu. Rev. Cell Dev. Biol. 19:
237-259.
online Kono T. et al. Birth of parthogenetic mice that can develop to adulthood. Nature, 42: 860-864 (2004). |