BCH 6744C: Molecular Structure Determination by X-ray Crystallography


Course Organizer: R. McKenna & M.Agbandje-McKenna


Course Description: 1-2 credit, Fall  semester

Objective is to provide detailed theoretical and practical instructions on the methodology of X-ray
crystallography. Students will learn the theory  behind the technique of X-ray crystallography and will
apply the knowledge obtained to the three-dimensional structure determination of a macromolecule (
lysozyme). The laboratory class will provide practical experience in sample preparation, operation of
the instrumentation, data acquisition, data analysis, phasing and refinement. The anticipation is that
students will take both the theoretical and practical sections of the course for 2 credits, which will run
concurrently. However, students may earn 1 credit by either attending the lectures on the theory of
X-ray crystallography or the laboratory class. This hands-on approach will reinforce the applicability of
this methodology in the analysis of the functional properties of a biological macromolecule.

15 hours of lectures on X-ray crystallography
15 hour of lab/applications