Advanced Concentration

Biochemistry & Molecular Biology

Mentor

Robert McKenna, Ph.D.

Department

Biochemistry & Molecular Biology

Enrolled

August 2002

Lab Room

LG-171

Lab Phone

846-2001

E-mail

zoefish@ufl.edu

Education

B.S., Biochemistry, University of Stellenbosch (South Africa)

Dissertation Research

Structural insights into the active site of alpha-carbonic anhydrases

Publications

Fisher SZ, Tariku I, Case NM, Tu C, Seron T, Silverman DN, Linser PJ, McKenna R.  Expression, purification, kinetic, and structural characterization of an alpha-class carbonic anhydrase from Aedes aegypti (AaCA1). Biochim Biophys Acta. 2006 Aug;1764(8):1413-9. abstract

Fisher SZ, Govindasamy L, Boyle N, Agbandje-McKenna M, Silverman DN, Blackburn GM, McKenna R.  X-ray crystallographic studies reveal that the incorporation of spacer groups in carbonic anhydrase inhibitors causes alternate binding modes. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt 7):618-22.  abstract

Fisher SZ, Govindasamy L, Tu C, Agbandje-McKenna M, Silverman DN, Rajaniemi HJ, McKenna R.  Structure of human salivary alpha-amylase crystallized in a C-centered monoclinic space group. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt 2):88-93. Epub 2006 Jan 27. abstract

Budayova-Spano M, Fisher SZ, Dauvergne MT, Agbandje-McKenna M, Silverman DN, Myles DA, McKenna R.  Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II.
Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Jan 1;62(Pt 1):6-9. Epub 2005 Dec 16. abstract

Clemente JC, Govindasamy L, Madabushi A, Fisher SZ, Moose RE, Yowell CA, Hidaka K, Kimura T, Hayashi Y, Kiso Y, Agbandje-McKenna M, Dame JB, Dunn BM, McKenna R. Structure of the aspartic protease plasmepsin 4 from the malarial parasite Plasmodium malariae bound to an allophenylnorstatine-based inhibitor.
Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):246-52. Epub 2006 Feb 22. abstract

Bhatt D, Tu C, Fisher SZ, Hernandez Prada JA, McKenna R, Silverman DN. Proton transfer in a Thr200His mutant of human carbonic anhydrase II. Proteins. 2005 Nov 1;61(2):239-45.  abstract

Duda DM, Tu C, Fisher SZ, An H, Yoshioka C, Govindasamy L, Laipis PJ, Agbandje-McKenna M, Silverman DN, McKenna R. Human carbonic anhydrase III: structural and kinetic study of catalysis and proton transfer. Biochemistry. 2005 Aug 2;44(30):10046-53.  abstract

Madabushi A, Chakraborty S, Fisher SZ, Clemente JC, Yowell C, Agbandje-McKenna M, Dame JB, Dunn BM, McKenna R. Crystallization and preliminary X-ray analysis of the aspartic protease plasmepsin 4 from the malarial parasite Plasmodium malariae. Acta Cryst. 2005; F61, 228-231.

Fisher Z, Hernandez Prada JA, Tu C, Duda D, Yoshioka C, An H, Govindasamy L, Silverman DN, McKenna R.  Structural and kinetic characterization of active-site histidine as a proton shuttle in catalysis by human carbonic anhydrase II. Biochemistry. 2005 Feb 1;44(4):1097-105.  abstract

Ketcham C, Wang F, Fisher SZ, Ercan A, Van Der Wel H, Locke RD, Ud-Doulah k S, Matta KL, West CM.  Specificity of a soluble UDP-galactose:fucoside a1,3 galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in dictyostelium. J Biol Chem. 2004 Jul 9;279(28):29050-9.  abstract

Van Der Wel H, Fisher SZ, West CM.  A bifunctional diglycosyltransferase forms the Fucalpha1,2Galbeta1,3-disaccharide on Skp1 in the cytoplasm of dictyostelium. J Biol Chem. 2002 Nov 29;277(48):46527-34.  abstract

Abstracts Presented at Meetings

Conference on New Frontiers in Neutron Macromolecular Crystallography 2005 in Oakridge, TN.  Proposed Neutron Diffraction Studies to Elucidate the Proton Transfer Mechanism via Hydrogen Bonded Networks in Carbonic Anhydrase and Superoxide Dismutase. S.Z. Fisher, P. Quint, R. Reutzel, D. Bhatt, K. Weiss, M. Budayova-Spano, F. Meilleur, C.K. Tu, L. Govindasamy, M. Agbandje-McKenna, D.N. Silverman, D.A.A. Myles, R. McKenna.

American Crystallographic Association 2005, Orlando, FL.  Structural studies of Human Carbonic Anhydrase II in Complex with 3 Novel Inhibitors. S.Z. Fisher, L. Govindasamy, M. Agbandje-McKenna, R. McKenna.

American Crystallographic Association 2004, Chicago, IL.  Proton Transfer in Human Carbonic Anhydrase II: Effects of pH and position of the proton shuttle on the transfer rate. S.Z. Fisher, D. Bhatt, J. Hernandez, C.K. Tu, D.M. Duda, C. Yoshioka, H. An, L. Govindasamy, D.N. Silverman, R. McKenna.

International Carbonic Anhydrase Meeting 2003, Smolenice, Slovakia.  Comparative computer modeling of the 14 human carbonic anhydrase domains. S.Z. Fisher, C.K. Tu, L. Govindasamy, D.N. Silverman, R. McKenna.

Honors & Awards

University of Florida Outstanding Student Award, 2003.

University of Florida Outstanding International Student Award, 2004.