Physiology & Pharmacology Alumni


Biochemistry & Molecular Biology

Immunology & Microbiology

Neuroscience

Genetics

Molecular Cell Biology

Physiology & Pharmacology


Bowman

Bui

Cao

Chen

Davis

Elder

Evans

Fleegal

Gardon

Giroux

Greenleaf

Handayani

Hearn

Hua

Huentelman

LeFrancois

Li G

Li L

McGarry

Metcalfe

Pan

Placzek

Reimsnider

Sabo-Attwood

Samedi

Sandberg

Schaub

Sellers

Sengupta

Shi

Tang

Taylor

Tolson

Wilsey

Wallace

Wang

Zhang

Christopher J. Bowman, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Nancy D. Denslow, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1996 - May 2002

Dissertation Title

Gene regulation in teleosts by estradiol and estrogen mimics

Professional Positions

CIIT Centers for Health Research, Research Triangle Park, NC

Education

B.S., University of Florida

Publications

Bowman CJ, Barlow NJ, Turner KJ, Wallace DG, Foster PM.  Effects of in utero exposure to finasteride on androgen-dependent reproductive development in the male rat. Toxicol Sci. 2003 Aug;74(2):393-406.   abstract

Turner KJ, McIntyre BS, Phillips SL, Barlow NJ, Bowman CJ, Foster PM.  Altered gene expression during rat Wolffian duct development in response to in utero exposure to the antiandrogen linuron. Toxicol Sci. 2003 Jul;74(1):114-28.   abstract

Bowman CJ, Kroll KJ, Gross TG, Denslow ND.  Estradiol-induced gene expression in largemouth bass (Micropterus salmoides). Mol Cell Endocrinol. 2002 Oct 31;196(1-2):67-77.   abstract

Hemmer MJ, Bowman CJ, Hemmer BL, Friedman SD, Marcovich D, Kroll KJ, Denslow ND.  Vitellogenin mRNA regulation and plasma clearance in male sheepshead minnows, (Cyprinodon variegatus) after cessation of exposure to 17 beta-estradiol and p-nonylphenol. Aquat Toxicol. 2002 Jul;58(1-2):99-112.   abstract

Denslow ND, Lee HS, Bowman CJ, Hemmer MJ, Folmar LC.  Multiple responses in gene expression in fish treated with estrogen. Comp Biochem Physiol B Biochem Mol Biol. 2001 Jun;129(2-3):277-82.  abstract

Hemmer MJ, Hemmer BL, Bowman CJ, Kroll KJ, Folmar LC, Marcovich D, Hoglund MD, Denslow ND.  Effects of p-nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus). Environ Toxicol Chem. 2001 Feb;20(2):336-43.  abstract

Denslow ND, Bowman CJ, Ferguson RJ, Lee HS, Hemmer MJ, Folmar LC.  Induction of gene expression in sheepshead minnows (Cyprinodon variegatus) treated with 17beta-estradiol, diethylstilbestrol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation. Gen Comp Endocrinol. 2001 Mar;121(3):250-60.  abstract

Bowman CJ, Kroll KJ, Hemmer MJ, Folmar LC, Denslow ND.  Estrogen-induced vitellogenin mRNA and protein in sheepshead minnow (Cyprinodon variegatus). Gen Comp Endocrinol. 2000 Dec;120(3):300-13.  abstract

Funkenstein B, Bowman CJ, Denslow ND, Cardinali M, Carnevali O.  Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata. Mol Cell Endocrinol. 2000 Sep 25;167(1-2):33-41.  abstract

Folmar LC, Hemmer M, Hemmer R, Bowman C, Kroll K, Denslow ND.  Comparative estrogenicity of estradiol, ethynyl estradiol and diethylstilbestrol in an in vivo, male sheepshead minnow (Cyprinodon variegatus), vitellogenin bioassay. Aquat Toxicol. 2000 May 1;49(1-2):77-88.  abstract

Honors & Awards

Graduate Fellowship for Outstanding Reseach, UF College of Medicine, 2000


Jonathan D. Bui, M.D., Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

M. Ian Phillips, Ph.D. & Stephen J. Blackband, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1996 - August 2000 (Ph.D.)

Dissertation Title

Quantitative NMR studies of perfused brain slices

COS Profile

http://expertise.cos.com/cgi-bin/exp.cgi?id=223215

Professional Positions

Clinical Fellow, Harvard University, Beth Israel, Medicine

Education

B.S., Zoology, B.S., Biophysical Chemistry and Molecular Biology, University of Florida

Publications

Zhang YC, Bui JD, Shen L, Phillips MI.  Antisense inhibition of beta(1)-adrenergic receptor mRNA in a single dose produces a profound and prolonged reduction in high blood pressure in spontaneously hypertensive rats. Circulation. 2000 Feb 15;101(6):682-8.  abstract

Forder JR, Bui JD, Buckley DL, Blackband SJ.  MR imaging measurement of compartmental water diffusion in perfused heart slices. Am J Physiol Heart Circ Physiol. 2001 Sep;281(3):H1280-5.  abstract

Hsu EW, Buckley DL, Bui JD, Blackband SJ, Forder JR.  Two-component diffusion tensor MRI of isolated perfused hearts. Magn Reson Med. 2001 Jun;45(6):1039-45.  abstract

Shi J, Bui JD, Yang SH, He Z, Lucas TH, Buckley DL, Blackband SJ, King MA, Day AL, Simpkins JW.  Estrogens decrease reperfusion-associated cortical ischemic damage: an MRI analysis in a transient focal ischemia model. Stroke. 2001 Apr;32(4):987-92.  abstract

Zhang YC, Bui JD, Shen L, Phillips MI.  Antisense inhibition of beta(1)-adrenergic receptor mRNA in a single dose produces a profound and prolonged reduction in high blood pressure in spontaneously hypertensive rats. Circulation. 2000 Feb 15;101(6):682-8.  abstract

Blackband SJ, Buckley DL, Bui JD, Phillips MI.  NMR microscopy--beginnings and new directions. MAGMA. 1999 Dec;9(3):112-6. Review.  abstract

Bui JD, Zelles T, Lou HJ, Gallion VL, Phillips MI, Tan W.  Probing intracellular dynamics in living cells with near-field optics. J Neurosci Methods. 1999 Jul 1;89(1):9-15.  abstract

Buckley DL, Bui JD, Phillips MI, Blackband SJ.  MRI measurement of cell volume fraction in the perfused rat hippocampal slice. Magn Reson Med. 1999 Sep;42(3):603-7.  abstract

Bui JD, Buckley DL, Phillips MI, Blackband SJ.  Nuclear magnetic resonance imaging measurements of water diffusion in the perfused hippocampal slice during N-methyl-D-aspartate-induced excitotoxicity. Neuroscience. 1999;93(2):487-90.  abstract

Bui JD, Nammari DR, Buckley DL, Inglis BA, Silver XS, Mareci TH, Phillips MI.  In vivo dynamics and distribution of intracerebroventricularly administered gadodiamide, visualized by magnetic resonance imaging. Neuroscience. 1999 Mar;90(3):1115-22.  abstract

Buckley DL, Bui JD, Phillips MI, Zelles T, Inglis BA, Plant HD, Blackband SJ.  The effect of ouabain on water diffusion in the rat hippocampal slice measured by high resolution NMR imaging. Magn Reson Med. 1999 Jan;41(1):137-42.  abstract

Blackband SJ, Bui JD, Buckley DL, Zelles T, Plant HD, Inglis BA, Phillips MI. MR microscopy of perfused brain slices. Magn Reson Med. 1997 Dec;38(6):1012-5.  abstract

Bui JD, Kimura B, Phillips MI.  Losartan potassium, a nonpeptide antagonist of angiotensin II, chronically administered p.o. does not readily cross the blood-brain barrier. Eur J Pharmacol. 1992 Aug 14;219(1):147-51.  abstract


Xuefang Cao, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

William Stratford May, Jr., M.D., Ph.D. 

Department

Physiology & Functional Genomics

Enrolled

August 1999 - December 2003

Dissertation Title

Cleavage of Bas to Pis Bax facilitates stress-induced apoptosis

Professional Positions

Education

Publications

Cao X, Deng X, May WS.  Cleavage of Bax to p18 Bax accelerates stress-induced apoptosis, and a cathepsin-like protease may rapidly degrade p18 Bax. Blood. 2003 Oct 1;102(7):2605-14.  abstract


Shuzhen Chen, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Jeffrey K. Harrison, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1999 - May 2004

Dissertation Title

TGF-beta-1 regulation of chemokine receptors in rat microglia and human macrophages  (full text)

Professional Positions

Postdoctoral Associate, Harvard Medical School

Education

B.S., College Of Life Sciences, Beijing University 

Publications

Davis CN, Chen S, Boehme SA, Bacon KB, Harrison JK.  Chemokine receptor binding and signal transduction in native cells of the central nervous system. Methods. 2003 Apr;29(4):326-34.  abstract

Chen S, Luo D, Streit WJ, Harrison JK.  TGF-beta1 upregulates CX3CR1 expression and inhibits fractalkine-stimulated signaling in rat microglia. J Neuroimmunol. 2002 Dec;133(1-2):46-55.  abstract

Harrison JK, Fong AM, Swain PA, Chen S, Yu YR, Salafranca MN, Greenleaf WB, Imai T, Patel DD.  Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion.  J Biol Chem. 2001 Jun 15;276(24):21632-41.  abstract

Honors & Awards

Best Thesis of the Department of Cell Biology and Genetics, Beijing University, 1999

Gangsong Scholarshop, Beijing University, 1998

Xiyue Scholarship, Beijing University, 1997

Tan Jiazhen (C.C. Tan) Life Science Scholarship


Christopher N. Davis, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Jeffrey K. Harrison, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1999 - August 2004

Dissertation Title

Mammalian and viral chemokines provide insight into the mechanism of chemokine receptor activation  (full text)

Professional Positions

Postdoctoral Associate, Dept. of Neuropharmacology, The Scripps Research Institute, San Diego, CA

Education

B.S., Neurobiological Sciences and Psychology, University of Florida

Publications

Streit WJ, Davis CN, Harrison JK.  Role of fractalkine (CX3CL1) in regulating neuron-microglia interactions: development of viral-based CX3CR1 antagonists. Curr Alzheimer Res. 2005 Apr;2(2):187-9.  abstract

Davis CN, Zujovic V, Harrison JK.  vMIP-II and fractalkine (CX3CL1) chimeras identify molecular determinants of affinity, efficacy, and selectivity at CX3CR1. Mol Pharmacol. 2004 Dec;66(6):1431-9.  abstract

Davis CN, Chen S, Boehme SA, Bacon KB, Harrison JK. Chemokine receptor binding and signal transduction in native cells of the central nervous system. Methods. 2003 Apr;29(4):326-34.  abstract


Ileana Montanez-Clemente Elder, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

David N. Silverman, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 2000 - August 2004

Dissertation Title

Kinetic and structural studies on the activation of the proton transfer in catalysis by carbonic anhydrase

Professional Positions

Education

B.S., Industrial Chemistry, University of Puerto Rico

Publications

Elder I, Han S, Tu C, Steele H, Laipis PJ, Viola RE, Silverman DN. Activation of carbonic anhydrase II by active-site incorporation of histidine analogs. Arch Biochem Biophys. 2004 Jan 15;421(2):283-9.  abstract

An H, Tu C, Duda D, Montanez-Clemente I, Math K, Laipis PJ, McKenna R, Silverman DN. Chemical rescue in catalysis by human carbonic anhydrases II and III. Biochemistry. 2002 Mar 5;41(9):3235-42.  abstract

Montanez-Clemente I, Alvira E, Macias M, Ferrer A, Fonceca M, Rodriguez J, Gonzalez A, Barletta G. Enzyme activation in organic solvents: co-lyophilization of subtilisin Carlsberg with methyl-beta-cyclodextrin renders an enzyme catalyst more active than the cross-linked enzyme crystals. Biotechnol Bioeng. 2002 Apr 5;78(1):53-9.  abstract

Martinez SG, Alvira E, Cordero LV, Ferrer A, Montanes-Clemente I, Barletta G. High initial activity but low storage stability observed for several preparations of subtilisin Carslberg suspended in organic solvents. Biotechnol Prog. 2002 Nov-Dec;18(6):1462-6.  abstract

Santos AM, Montañez Clemente I, Barletta G, Griebenow K. Activation of serine protease subtilisin Carlsberg in organic solvents: combined effect of methyl-ß-cyclodextrin and water. Biotech Letters. 1999 Dec;21(12):1113-8.

Griebenow K, Laureano YD, Santos AM, Clemente IM, Rodriguez L, Vidal MW, Barletta G. Improved enzyme activity and enantioselectivity in organic solvents by methyl--cyclodextrin. J Am Chem Soc. 1999;121(36); 8157-8163.


Jenafer Evans, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

C. H. Gelband, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1996 - May 2001

Dissertation Title

Angiotensin II modulation of neuronal calcium current  (full text)

Professional Positions

Postdoctoral Associate, Calcium Signals Laboratory (David Yue, M.D., Ph.D.), Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD

Education

B.S., Chemistry, University of Virginia

Publications

Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, Shaw G.  Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J Neurophysiol. 2002 Feb;87(2):1076-85.  abstract

Evans J, Gelband CH. A novel Ca2+ channel in vascular smooth muscle? Circ Res. 1999 Oct 1;85(7):651-2. Review.  abstract

Honors & Awards

Outstanding Chemistry Student Award, University of Virginia, 1996


Melissa A. Fleegal, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Colin Sumners, Ph.D. 

Department

Physiology & Functional Genomics

Enrolled

August 1997 - August 2002

Dissertation Title

Angiotensin II modulation of neuronal intracellular signaling cascades regulates short-term and long-term physiological responses  (full text)

Professional Positions

Education

B.S., Biology, Lebanon Valley College, PA

Publications

Fleegal MA, Sumners C.  Angiotensin II induction of AP-1 in neurons requires stimulation of PI3-K and JNK. Biochem Biophys Res Commun. 2003 Oct 17;310(2):470-7.  abstract

Fleegal MA, Sumners C.  Drinking behavior elicited by central injection of angiotensin II: roles for protein kinase C and Ca2+/calmodulin-dependent protein kinase II. Am J Physiol Regul Integr Comp Physiol. 2003 Sep;285(3):R632-40.  abstract

Marcus JS, Karackattu SL, Fleegal MA, Sumners C.  Cytokine-stimulated inducible nitric oxide synthase expression in astroglia: role of Erk mitogen-activated protein kinase and NF-kappaB. Glia. 2003 Jan 15;41(2):152-60.  abstract

Sumners C, Fleegal MA, Zhu M.  Angiotensin AT1 receptor signalling pathways in neurons. Clin Exp Pharmacol Physiol. 2002 May-Jun;29(5-6):483-90. Review.  abstract

Busche S, Gallinat S, Fleegal MA, Raizada MK, Sumners C.  Novel role of macrophage migration inhibitory factor in angiotensin II regulation of neuromodulation in rat brain. Endocrinology. 2001 Nov;142(11):4623-30.  abstract

Baghdoyan HA, Lydic R, Fleegal MA.  M2 muscarinic autoreceptors modulate acetylcholine release in the medial pontine reticular formation. J Pharmacol Exp Ther. 1998 Sep;286(3):1446-52.  abstract

Roth MT, Fleegal MA, Lydic R, Baghdoyan HA.  Pontine acetylcholine release is regulated by muscarinic autoreceptors. Neuroreport. 1996 Nov 25;7(18):3069-72.  abstract

Honors & Awards

Graduate Fellowship for Outstanding Reseach, UF College of Medicine, 2000


Monica L. Gardon, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Harm J. Knot, Pharm.D., Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1996 - December 2003

Dissertation Title

Functional remodeling of the renal and cerebral resistance vasculature in hypertensive rats

Professional Positions

Education

Publications

Gardon M, Raizada MK, Katovich MJ, Berecek KH, Gelband CH.  Gene therapy for hypertension and restenosis. J Renin Angiotensin Aldosterone Syst. 2000 Sep;1(3):211-6.  Review. 

Gardon ML, Gelband CH, Katovich MJ, Raizada MK.  The potential use of gene therapy in the control of hypertension. Drugs Today (Barc). 1999 Dec;35(12):925-30.  abstract


Damian Giroux, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Charles E. Wood, Ph.D. 

Department

Physiology & Functional Genomics

Enrolled

August 1998 - May 2002

Dissertation Title

Estrogen influences on fetal CNS cyclooxygenase and nitric oxide synthase isoforms in response to cerebral hypoperfusion

Professional Positions

Education

M.S., University of Florida

Publications

Wood CE, Giroux D, Gridley K.  Fetal brain regional responses to cerebral hypoperfusion: modulation by estrogen. Brain Res. 2003 Dec 12;993(1-2):84-9.  abstract

Wood CE, Giroux D.  Central nervous system prostaglandin endoperoxide synthase-1 and -2 responses to oestradiol and cerebral hypoperfusion in late-gestation fetal sheep. J Physiol. 2003 Jun 1;549(Pt 2):573-81.  abstract

Deauseault D, Giroux D, Wood CE.  Ontogeny of immunoreactive prostaglandin endoperoxide synthase isoforms in ovine fetal pituitary, hypothalamus and brainstem. Neuroendocrinology. 2000 May;71(5):287-91.  abstract


William B. Greenleaf, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

David N. Silverman, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1999 - May 2004

Dissertation Title

Proton transfer in catalysis by iron and manganese superoxide dismutase

Professional Positions

Postdoctaral Associate, University of Colorado Health Science Center

Education

Publications

Greenleaf WB, Perry JJ, Hearn AS, Cabelli DE, Lepock JR, Stroupe ME, Tainer JA, Nick HS, Silverman DN.  Role of hydrogen bonding in the active site of human manganese superoxide dismutase. Biochemistry. 2004 Jun 8;43(22):7038-45.  abstract

Hearn AS, Fan L, Lepock JR, Luba JP, Greenleaf WB, Cabelli DE, Tainer JA, Nick HS, Silverman DN.  Amino acid substitution at the dimeric interface of human manganese superoxide dismutase. J Biol Chem. 2004 Feb 13;279(7):5861-6.  abstract

Greenleaf WB, Silverman DN.  Activation of the proton transfer pathway in catalysis by iron superoxide dismutase. J Biol Chem. 2002 Dec 20;277(51):49282-6.  abstract

Honors and Awards

Alumni Graduate Fellowship, University of Florida

Mercz Index Award, Florida State University

Phi Beta Kappa, Alpha of Florida Chapter, Florida State University

Golden Key National Honor Society, Florida State University

National Merit Scholarship, National Merit Corporation, Florida State University

National Merit Scholarship, Florida State University


Renita Handayani, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Kathleen T. Shiverick, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 2000 - December 2004

Dissertation Title

Prospective cancer chemoprevention agents:  Mechanisms of the effects of soy isoflavone and vitamin E on the regulation of p21 (WAF1/CIP1) gene expression

Professional Positions

Education

B.S., Pharmacy

Publications

Ason B, Handayani R, Williams CR, Bertram JG, Hingorani MM, O'Donnell M, Goodman MF, Bloom LB. Mechanism of loading the Escherichia coli DNA polymerase III beta sliding clamp on DNA. Bona fide primer/templates preferentially trigger the gamma complex to hydrolyze ATP and load the clamp. J Biol Chem. 2003 Mar 21;278(12):10033-40.  abstract


Amy S. Hearn, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

David N. Silverman, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1996 - May 2001

Dissertation Title

Product inhibition in human manganese superoxide dismutase

Professional Positions

Postdoctoral Associate, American Lung Association Fellow, Dept. Neuroscience, UF College of Medicine

Education

B.S., University of Florida

Publications

Greenleaf WB, Perry JJ, Hearn AS, Cabelli DE, Lepock JR, Stroupe ME, Tainer JA, Nick HS, Silverman DN.  Role of hydrogen bonding in the active site of human manganese superoxide dismutase. Biochemistry. 2004 Jun 8;43(22):7038-45.  abstract

Davis CA, Hearn AS, Fletcher B, Bickford J, Garcia JE, Leveque V, Melendez JA, Silverman DN, Zucali J, Agarwal A, Nick HS.  Potent anti-tumor effects of an active site mutant of human manganese-superoxide dismutase. Evolutionary conservation of product inhibition. J Biol Chem. 2004 Mar 26;279(13):12769-76.  abstract

Hearn AS, Fan L, Lepock JR, Luba JP, Greenleaf WB, Cabelli DE, Tainer JA, Nick HS, Silverman DN.  Amino acid substitution at the dimeric interface of human manganese superoxide dismutase. J Biol Chem. 2004 Feb 13;279(7):5861-6.  abstract

Hearn AS, Stroupe ME, Cabelli DE, Ramilo CA, Luba JP, Tainer JA, Nick HS, Silverman DN.  Catalytic and structural effects of amino acid substitution at histidine 30 in human manganese superoxide dismutase: insertion of valine C gamma into the substrate access channel. Biochemistry. 2003 Mar 18;42(10):2781-9.  abstract

Hearn AS, Stroupe ME, Cabelli DE, Lepock JR, Tainer JA, Nick HS, Silverman DN.  Kinetic analysis of product inhibition in human manganese superoxide dismutase. Biochemistry. 2001 Oct 9;40(40):12051-8.  abstract

Cabelli DE, Guan Y, Leveque V, Hearn AS, Tainer JA, Nick HS, Silverman DN.  Role of tryptophan 161 in catalysis by human manganese superoxide dismutase. Biochemistry. 1999 Sep 7;38(36):11686-92.  abstract

Hearn AS, Tu C, Nick HS, Silverman DN.  Characterization of the product-inhibited complex in catalysis by human manganese superoxide dismutase. J Biol Chem. 1999 Aug 27;274(35):24457-60.  abstract


Yi Hua, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Charles E. Wood, Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled

August 1998 - December 2002

Dissertation Title

Progesterone and corticosteroid interaction during pregnancy in ewes

Professional Positions

Education

Publications

Richards EM, Hua Y, Keller-Wood M.  Pharmacology and physiology of ovine corticosteroid receptors. Neuroendocrinology. 2003 Jan;77(1):2-14.  abstract


Matthew J. Huentelman, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Mohan K. Raizada, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1998 - December 2003

Dissertation Title

HIV-1 based viral vector development for gene transfer to the cardiovascular system  (full text)

Professional Positions

Education

B.S., Ohio State University, Columbus, OH

Publications

Huentelman MJ, Zubcevic J, Hernandez Prada JA, Xiao X, Dimitrov DS, Raizada MK, Ostrov DA.  Structure-Based Discovery of a Novel Angiotensin-Converting Enzyme 2 Inhibitor. Hypertension. 2004 Oct 18;44:1-4.  abstract

Metcalfe BL, Huentelman MJ, Parilak LD, Taylor DG, Katovich MJ, Knot HJ, Sumners C, Raizada MK.  Prevention of cardiac hypertrophy by angiotensin II type-2 receptor gene transfer. Hypertension. 2004 Jun;43(6):1233-8.  abstract

Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK.  Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Physiol Genomics. 2003 Feb 6;12(3):221-8.  abstract

Huentelman MJ, Reaves PY, Katovich MJ, Raizada MK.  Large-scale production of retroviral vectors for systemic gene delivery. Methods Enzymol. 2002;346:562-73.

Metcalfe BL, Sellers KW, Jeng MJ, Huentelman MJ, Katovich MJ, Raizada MK.  Gene therapy for cardiovascular disorders: is there a future? Ann N Y Acad Sci. 2001 Dec;953:31-42. Review.  abstract

Pachori AS, Huentelman MJ, Francis SC, Gelband CH, Katovich MJ, Raizada MK.  The future of hypertension therapy: sense, antisense, or nonsense? Hypertension. 2001 Feb;37(2 Part 2):357-64. Review.  abstract


Susan E. LeFrancois, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

William R. Kem, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 2000 - December 2004

Dissertation Title

A Structure Activity Investigation of Benzylidene Anabaseine Interactions with the Alpha7 Nicotinic Acetylcholine Receptor

Professional Positions

Education

B.S., University of South Florida

Publications

Kem WR, Wildeboer K, LeFrancois S, Raja M, Marszalec W, Braekman JC.  Nicotinic receptor inhibition by Tetraponera ant alkaloids. Cell Mol Neurobiol. 2004 Aug;24(4):535-51.  abstract

Kem WR, Mahnir VM, Prokai L, Papke RL, Cao X, LeFrancois S, Wildeboer K, Prokai-Tatrai K, Porter-Papke J, Soti F. Hydroxy metabolites of the Alzheimer's drug candidate 3-[(2,4-dimethoxy)benzylidene]-anabaseine dihydrochloride (GTS-21): their molecular properties, interactions with brain nicotinic receptors, and brain penetration. Mol Pharmacol. 2004 Jan;65(1):56-67.  abstract


Gang Li, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Philip Scarpace, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1999 - August 2003

Dissertation Title

Effects of the melanocortin agonist melanotan II, central pro-opiomelanocortin and interleukin-6 gene therapies on the regulation of body weight and energy homeostasis  (full text)

Professional Positions

Education

B.Med., Zhejiang University School of Medicine, P.R. China

M.D., Peking Union Medical College, P.R. China

Publications

Li G, Zhang Y, Wilsey JT, Scarpace PJ.  Unabated anorexic and enhanced thermogenic responses to melanotan II in diet-induced obese rats despite reduced melanocortin 3 and 4 receptor expression. J Endocrinol. 2004 Jul;182(1):123-32.  abstract

Li G, Mobbs CV, Scarpace PJ.  Central pro-opiomelanocortin gene delivery results in hypophagia, reduced visceral adiposity, and improved insulin sensitivity in genetically obese Zucker rats. Diabetes. 2003 Aug;52(8):1951-7.  abstract

Li G, Klein RL, Matheny M, King MA, Meyer EM, Scarpace PJ.  Induction of uncoupling protein 1 by central interleukin-6 gene delivery is dependent on sympathetic innervation of brown adipose tissue and underlies one mechanism of body weight reduction in rats. Neuroscience. 2002;115(3):879-89.  abstract


Lingyun Li, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Dietmar W. Siemann, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1996 - December 2000

Dissertation Title

Studies of the anticancer effects of the vascular targeting agent combretastatin A-4 disodium phosphate (CA4DP)

Professional Positions

Education

Publications

Rojiani AM, Li L, Rise L, Siemann DW.  Activity of the vascular targeting agent combretastatin A-4 disodium phosphate in a xenograft model of AIDS-associated Kaposi's sarcoma. Acta Oncol. 2002;41(1):98-105.  abstract

Li L, Rojiani AM, Siemann DW.  Preclinical evaluations of therapies combining the vascular targeting agent combretastatin A-4 disodium phosphate and conventional anticancer therapies in the treatment of Kaposi's sarcoma. Acta Oncol. 2002;41(1):91-7.  abstract

Li L, Rojiani A, Siemann DW.  Targeting the tumor vasculature with combretastatin A-4 disodium phosphate: effects on radiation therapy. Int J Radiat Oncol Biol Phys. 1998 Nov 1;42(4):899-903.  abstract


Michelle A. McGarry, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Kathleen T. Shiverick, Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled

August 1997 - August 2001

Dissertation Title

Benzo(A) pyrene-mediated effects on cellular adhesion and signal transduction pathways in endometrial cancer

Professional Positions

Resident, Dept. of Pediatrics, University of Chicago

Medical Student, University of Florida College of Medicine

Education

Publications

McGarry MA, Charles GD, Medrano T, Bubb MR, Grant MB, Campbell-Thompson M, Shiverick KT.  Benzo(a)pyrene, but not 2,3,7,8-tetrachlorodibenzo-p-dioxin, alters cell adhesion proteins in human uterine RL95-2 cells. Biochem Biophys Res Commun. 2002 May 31;294(1):101-7.  abstract


Beverly L. Metcalfe, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Mohan K. Raizada, Ph.D. 

Department

Physiology & Functional Genomics

Enrolled

August 1999 - May 2004

Dissertation Title

Defining the role of the angiotensin II type 2 receptor in cardiovascular disease

Professional Positions

Postdoctoral Associate, University of Florida College of Medicine, Dept. of Physiology & Functional Genomics

Education

Publications

Metcalfe BL, Huentelman MJ, Parilak LD, Taylor DG, Katovich MJ, Knot HJ, Sumners C, Raizada MK.  Prevention of cardiac hypertrophy by angiotensin II type-2 receptor gene transfer. Hypertension. 2004 Jun;43(6):1233-8.  abstract

Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK.  Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Physiol Genomics. 2003 Feb 6;12(3):221-8. Epub 2002 Dec 10.  abstract

Metcalfe BL, Sellers KW, Jeng MJ, Huentelman MJ, Katovich MJ, Raizada MK.  Gene therapy for cardiovascular disorders: is there a future? Ann N Y Acad Sci. 2001 Dec;953:31-42. Review.  abstract

Metcalfe BL, Raizada M, Katovich MJ.  Genetic targeting of the renin-angiotensin system for long-term control of hypertension. Curr Hypertens Rep. 2002 Feb;4(1):25-31. Review.  abstract


Hao Pan, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Maria Grant, M.D.  

Department

Pharmacology & Therapeutics

Enrolled

August 2001 - May 2006

Dissertation Title

Professional Positions

 

Education

B.S., Biochemistry, Nanjing University (P.R. China)

Publications

Grant MB, Afzal A, Spoerri P, Pan H, Shaw LC, Mames RN.  The role of growth factors in the pathogenesis of diabetic retinopathy. Expert Opin Investig Drugs. 2004 Oct;13(10):1275-93. abstract

Pan H, Nguyen NQ, Yoshida H, Bentzien F, Shaw LC, Rentier-Delrue F, Martial JA, Weiner R, Struman I, Grant MB.  Molecular targeting of antiangiogenic factor 16K hPRL inhibits oxygen-induced retinopathy in mice. Invest Ophthalmol Vis Sci. 2004 Jul;45(7):2413-9. abstract

Abstracts Presented at Meetings Through Graduate Studies

Honors & Awards


Andon Plazcek, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Roger L. Papke, Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled 

August 2000 - April 2005

Dissertation Title

Regulation of a7 Nicotinic Acetylcholine Receptor Function and Pharmacology by Amino Acid Sequence in the Second Transmembrane Domain

Education

B.S., University of South Florida

Professional Position   Postdoctoral Associate, UF Department of Pharmacology & Therapeutics

Publications

Placzek AN, Grassi F, Papke T, Meyer EM, Papke RL.  A single point mutation confers properties of the muscle-type nicotinic acetylcholine receptor to homomeric alpha7 receptors. Mol Pharmacol. 2004 Jul;66(1):169-77. abstract

Tan J, Town T, Abdullah L, Wu Y, Placzek A, Small B, Kroeger J, Crawford F, Richards D, Mullan M.  CD45 isoform alteration in CD4+ T cells as a potential diagnostic marker of Alzheimer's disease. J Neuroimmunol. 2002 Nov;132(1-2):164-72.  abstract

Papke RL, Horenstein BA, Placzek AN.  Inhibition of wild-type and mutant neuronal nicotinic acetylcholine receptors by local anesthetics. Mol Pharmacol. 2001 Dec;60(6):1365-74.  abstract

Suo Z, Su G, Placzek A, Kundtz A, Humphrey J, Crawford F, Mullan M.  A beta vasoactivity in vivo. Ann N Y Acad Sci. 2000 Apr;903:156-63.  abstract

Tan J, Town T, Placzek A, Kundtz A, Yu H, Mullan M.  Bcl-X(L) inhibits apoptosis and necrosis produced by Alzheimer's beta-amyloid1-40 peptide in PC12 cells. Neurosci Lett. 1999 Sep 3;272(1):5-8.  abstract

Tan J, Town T, Paris D, Placzek A, Parker T, Crawford F, Yu H, Humphrey J, Mullan M.  Activation of microglial cells by the CD40 pathway: relevance to multiple sclerosis. J Neuroimmunol. 1999 Jun 1;97(1-2):77-85.  abstract

Suo Z, Humphrey J, Kundtz A, Sethi F, Placzek A, Crawford F, Mullan M.  Soluble Alzheimers beta-amyloid constricts the cerebral vasculature in vivo. Neurosci Lett. 1998 Nov 27;257(2):77-80.  abstract

Suo Z, Tan J, Placzek A, Crawford F, Fang C, Mullan M.  Alzheimer's beta-amyloid peptides induce inflammatory cascade in human vascular cells: the roles of cytokines and CD40. Brain Res. 1998 Oct 5;807(1-2):110-7.  abstract

Abstracts Presented at Meetings

Meeting Society for Neuroscience 29th Annual Meeting, "CD45 isoform alterations in CD4+ T cells as a potential diagnostic marker of Alzheimers Disease", Miami Beach, FL


Sharon K. Reimsnider, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Charles E. Wood, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1997 - December 2003

Dissertation Title

Does cyclooxygenase modulate the fetal hypothalamic-pituitary-adrenal axis?  

Professional Positions

Education

Publications

Reimsnider SK, Wood CE.  Co-localisation of prostaglandin endoperoxide synthase and immunoreactive adrenocorticotrophic hormone in ovine foetal pituitary. J Endocrinol. 2004 Feb;180(2):303-10.  abstract

Reimsnider SK, Eckenrode SE, Marron MP, Muir A, She JX.  IL4 and IL4Ralpha genes are not linked or associated with type 1 diabetes. Pediatr Res. 2000 Feb;47(2):246-9.  abstract


Tara Sabo-Attwood, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Nancy D. Denslow, Ph.D.

Department

Biochemistry & Molecular Biology

Enrolled

August 1998 - August 2003

Dissertation Title

Characterization of estrogen receptors and estrogen receptor-regulated genes in largemouth bass

Professional Positions

Education

Publications

Sabo-Attwood T, Kroll KJ, Denslow ND.  Differential expression of largemouth bass (Micropterus salmoides) estrogen receptor isotypes alpha, beta, and gamma by estradiol. Mol Cell Endocrinol. 2004 Apr 15;218(1-2):107-18.  abstract

Larkin P, Sabo-Attwood T, Kelso J, Denslow ND.  Analysis of gene expression profiles in largemouth bass exposed to 17-beta-estradiol and to anthropogenic contaminants that behave as estrogens. Ecotoxicology. 2003 Dec;12(6):463-8.   abstract

Larkin P, Sabo-Attwood T, Kelso J, Denslow ND.  Gene expression analysis of largemouth bass exposed to estradiol, nonylphenol, and p,p'-DDE. Comp Biochem Physiol B Biochem Mol Biol. 2002 Dec;133(4):543-57.   abstract


Von G. Samedi, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Kathleen T. Shiverick, Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled

August 1997 - August 2001

Dissertation Title

Antiproliferative effects of isoflavonoids on prostate cancer cells and antitumorigenic effects in xenograft nude mice  

Professional Positions

Medical Student, University of Florida College of Medicine

Education

Publications

Rice L, Samedi VG, Medrano TA, Sweeney CA, Baker HV, Stenstrom A, Furman J, Shiverick KT.  Mechanisms of the growth inhibitory effects of the isoflavonoid biochanin A on LNCaP cells and xenografts. Prostate. 2002 Aug 1;52(3):201-12.  abstract


Eric M. Sandberg, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Peter P. Sayeski, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 2000 - August 2004 (degree conferred December 2004)

Dissertation Title

Jak2 tyrosine kinase:  New insights regarding structure, function, and pharmacology

Professional Positions

Postdoctoral Associate, Winship Cancer Center, Emory University, Atlanta, GA

Education

B.S., Microbiology & Cell Science, University of Florida

Publications

Sandberg EM, Ma X, VonDerLinden D, Godeny MD, Sayeski PP. Jak2 tyrosine kinase mediates angiotensin II-dependent inactivation of ERK2 via induction of mitogen-activated protein kinase phosphatase 1. J Biol Chem. 2004 Jan 16;279(3):1956-67.  abstract

VonDerLinden D, Ma X, Sandberg EM, Gernert K, Bernstein KE, Sayeski PP. Mutation of glutamic acid residue 1046 abolishes Jak2 tyrosine kinase activity. Mol Cell Biochem. 2002 Dec;241(1-2):87-94.  abstract

Sandberg EM, Sayeski PP.  Jak2 Tyrosine Kinase Mediates Oxidative Stress-induced Apoptosis in Vascular Smooth Muscle Cells. J Biol Chem. 2004 Aug 13;279(33):34547-52.  abstract

Sandberg EM, Wallace TA, Godeny MD, VonDerLinden D, Sayeski PP. (2004) Jak2 tyrosine kinase: A true Jak of all trades? (Invited review) Cell Biochem Biophys. In Press.

Sandberg EM, VonDerLinden D, Ostrov DA, Sayeski PP. (2004) Jak2 tyrosine kinase residues glutamic acid 1024 and arginine 1113 form a hydrogen bond interaction that is essential for Jak-STAT signal transduction. Mol Cell Biochem. In Press.

Abstracts Presented at Meetings during Graduate Studies

Sandberg EM, VonDerLinden D, Ostrov DA, Sayeski PP. Identification of a biochemical interaction between Jak2 tyrosine kinase residues glutamic acid 1024 and arginine 1113 that is essential for angiotensin II-dependent activation of the Jak-STAT signaling pathway. (2003) Experimental Biology, San Diego, CA (Abstract)

Sandberg EM, Ostrov DA, Sayeski PP. Structural analysis and ligand docking identifies a novel, potent small molecule inhibitor of Jak2 tyrosine kinase. (2004). Jaks and STATs: Development to Disease, British Colombia, Canada (Abstract)

Sandberg EM, Sayeski PP. Jak2 tyrosine kinase mediates oxidative stress-induced apoptosis in vascular smooth muscle cells. (2004) St. Jude's National Graduate Student Symposium, Memphis, TN (Oral Presentation)

Sandberg EM, Sayeski PP. Jak2 tyrosine kinase mediates oxidative stress-induced apoptosis in vascular smooth muscle cells. (June, 2004) European Society of Hypertension, Paris, France (Oral Presentation)

Honors & Awards

Professional memberships:  Alpha Zeta Professional Fraternity, Golden Key National Honor Society, American Physiological Society

2000: University of Florida Grinter Fellowship, $2000/year for 3 years

2002: University of Florida Medical Guild Research Grant, $500

2003: American Heart Association Predoctoral Fellowship, $19,000/year for 2 years

2004: Medical Guild Graduate Student Research Competition, 1st place in Physiology & Pharmacology, 3rd place overall

2004: 14th Meeting of the European Society of Hypertension Accommodation Grant


Christine Schaub, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Charles E. Wood, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 2001 - December 2005

Dissertation Title

The effects of estrogen on the ovine fetal hypothalamic-pituitary-adrenal axis

Professional Positions

Physiologist, U.S. Navy, Jan 2006

Education

B.S., Biology, Franklin and Marshall College

Publications

Abstracts Presented at Meetings Through Graduate Studies

Honors & Awards

Health Services Collegiate Program Navy Scholarship for Physiology


Kathleen W. Sellers, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Mohan K. Raizada, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 2000 - December 2004

Dissertation Title

Role of brain soluble epoxide hydrolase on blood pressure regulation

Professional Positions

Education

B.S., Biology,  Winthrop University

Publications

Veerasingham SJ, Sellers KW, Raizada MK. Related Articles, Links Functional genomics as an emerging strategy for the investigation of central mechanisms in experimental hypertension. Prog Biophys Mol Biol. 2004 Feb-Apr;84(2-3):107-23.  abstract

Yang H, Francis SC, Sellers K, DeBarros M, Sun C, Sumners C, Ferrario CM, Katovich MJ, Muro AF, Raizada MK. Hypertension-linked decrease in the expression of brain gamma-adducin. Circ Res. 2002 Oct 4;91(7):633-9.  abstract

Metcalfe BL, Sellers KW, Jeng MJ, Huentelman MJ, Katovich MJ, Raizada MK. Gene therapy for cardiovascular disorders: is there a future? Ann N Y Acad Sci. 2001 Dec;953:31-42.  abstract

Sellers KW, Katovich MJ, Gelband CH, Raizada MK. Gene therapy to control hypertension: current studies and future perspectives. Am J Med Sci. 2001 Jul;322(1):1-6.  abstract

Honors & Awards

American Heart Association Florida/Puerto Rico Affiliate Predoctoral Fellowship, 2003-2005

University of Florida Alumni Graduate Fellowship, 2000-2004

Honorary Mention, Beta Beta Beta Student Research Presentations, 2000

Trustees’ Scholarship, Winthrop University, 1996-2000

Abstracts Presented at Meetings Through Graduate Studies

Sun C, Sellers KW, Raizada MK, Sumners C. Angiotensin II-induced increases in neuronal firing and blood pressure: Role of ROS. 2004 Gordon Research Conference on Angiotensin.

Sellers KW, Veerasingham SJ, Francis SC, Raizada MK. Increased expression of soluble epoxide hydrolase in the brain of the spontaneously hypertensive rat. APS Meeting. 2003 FASEB J. 17: A1290, 823.5.  (oral presentation)

Sellers KW, Yang H, Boehlein SK, Schuster S, Ferrario C.M, Raizada, MK. Gene profiling reveals hypertension-associated decrease of Na+-K+-ATPase @ mRNA expression in the brain. APS Meeting. 2001 FASEB J. 632.6.  (oral presentation)


Nilanjana Sengupta, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Maria Grant, M.D.

Department

Pharmacology & Therapeutics

Enrolled 

August 2001 - April 2005

Dissertation Title

Education

B.S., Biology, University of South Carolina

Professional Position  Postdoctoral Fellow, Department of Ophthalmology, UF

Publications

Sengupta N, Caballero S, Mames RN, Timmers AM, Saban D, Grant MB.  Preventing stem cell incorporation into choroidal neovascularization by targeting homing and attachment factors. Invest Ophthalmol Vis Sci. 2005 Jan;46(1):343-8.  abstract

Sengupta N, Caballero S. Mouse models of visual deficits. Nat Med. 2004;10:663; author reply 663.

Caballero S, Sengupta N, Crafoord S, Lund R, Kruse FE, Young M, Grant MB. The many possible roles of stem cells in age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol. 2004 Jan;242(1):85-90.  abstract

Sengupta N, Caballero S, Mames RN, Butler JM, Scott EW, Grant MB. The role of adult bone marrow-derived stem cells in choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Nov;44(11):4908-13.  abstract

Abstracts Presented at Meetings

Grant MB, Caballero S, Sengupta N, Mames RN, Scott EW. Stem cell recruitment and participation in choroidal neovascularization following rupture of Bruch's membrane. In: First Workshop on Cell Transplantation in Age-Related Macular Degeneration. Cologne, Germany: Graefes Arch Clin Exp Ophthalmol; 2004:54-55.

Grant MB, Sengupta N, Caballero S, Mames RN, Guthrie SM, Scott EW. Hemangioblast activity of stem cells as promoted by injury and modulated by nitric oxide in a model of retinal neovascularization. In: 63rd Scientific Sessions of the American Diabetes Association. New Orleans, LA: Diabetes; 2003:A20.

Caballero S, Sengupta N, Mames RN, Scott EW, Grant MB. Stem cell recruitment and participation in choroidal neovascularization following rupture to Bruch’s membrane. Invest Ophthalmol Vis Sci 2003;44:ARVO Abstract 546.

Sengupta N, Caballero S, Scott EW, Mames RN, Guthrie, SM, Grant MB. Hemangioblast activity of stem cells as promoted by injury and modulated by nitric oxide in a model of retinal neovascularization. Invest Ophthalmol Vis Sci 2003;44:ARVO Abstract 2096.

Sengupta N, Caballero S, Mames RN, Scott EW, Grant MB. Preventing stem cell incorporation into aberrant ocular neovascularization. Keystone Symposia (Stem Cells), January 2004, Keystone, CO. Abstract 129.

Caballero S, Sengupta N, Mames RN, Scott EW, Grant MB. Hematopoietic stem cell contribution to rubeosis iridus. Keystone Symposia (Stem Cells), January 2004, Keystone, CO. Abstract 413.

Caballero S, Sengupta N, Mames RN, Scott EW, Grant MB. Hematopoietic Stem Cell Contribution to Neovascularization of the Iris. Invest Ophthalmol Vis Sci 2004;45:ARVO Abstract 502.

Sengupta N, Caballero S, Mames RN, Scott EW, Grant MB. Preventing Stem Cell Incorporation into Choroidal Neovascularization. Invest Ophthalmol Vis Sci 2004;45:ARVO Abstract 2233.  

Honors & Awards

National Eye Institute Travel Grant ($700) to attend and present a paper at the Annual Meeting (2004) of the Association for Research in Vision and Ophthalmology (ARVO).


Wenyin Shi, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Dietmar W. Siemann, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1998 - August 2002

Dissertation Title

Modulation of tumor angiogenesis through the use of antisense oligodeoxynucleotides targeted to VEGF and BFGF

Professional Positions

Postdoctoral Associate, University of Florida College of Medicine, Radiation Oncology

Education

Publications

Brazelle WD, Shi W, Siemann DW.  VEGF-associated tyrosine kinase inhibition increases the tumor response to single and fractionated dose radiotherapy. Int J Radiat Oncol Biol Phys. 2006 Jul 1;65(3):836-41.  abstract

Shi W, Siemann DW. S imultaneous targeting of VEGF message and VEGF receptor signaling as a therapeutic anticancer approach. Anticancer Res. 2004 Jan-Feb;24(1):213-8.  abstract

Shi W, Teschendorf C, Muzyczka N, Siemann DW.  Gene therapy delivery of endostatin enhances the treatment efficacy of radiation. Radiother Oncol. 2003 Jan;66(1):1-9.  abstract

Siemann DW, Shi W.  Targeting the tumor blood vessel network to enhance the efficacy of radiation therapy. Semin Radiat Oncol. 2003 Jan;13(1):53-61. Review.  abstract

Shi W, Siemann DW.  Inhibition of renal cell carcinoma angiogenesis and growth by antisense oligonucleotides targeting vascular endothelial growth factor. Br J Cancer. 2002 Jul 1;87(1):119-26.  abstract

Shi W, Teschendorf C, Muzyczka N, Siemann DW.  Adeno-associated virus-mediated gene transfer of endostatin inhibits angiogenesis and tumor growth in vivo. Cancer Gene Ther. 2002 Jun;9(6):513-21.  abstract

Honors & Awards

Graduate Fellowship for Outstanding Reseach, UF College of Medicine, 2001


Yi Tang, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

M. Ian Phillips, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 1999 - May 2003

Dissertation Title

Vigilant vector:  A hypoxia inducible and heart-specific double vector system for cardioprotection  
(full text)

Professional Positions

Education

B.Med., Clinical Medicine, Shanghai Medical University, Shanghai, P.R. China

Residency training, Department of Cardiology, Zhongshan Hospital, Shanghai Medical University, Shanghai, P.R. China

Publications

Tang YL, Tang Y, Zhang YC, Qian K, Shen L, Phillips MI.  Protection from ischemic heart injury by a vigilant heme oxygenase-1 plasmid system. Hypertension. 2004 Apr;43(4):746-51.  abstract

Tang YL, Zhao Q, Zhang YC, Cheng L, Liu M, Shi J, Yang YZ, Pan C, Ge J, Phillips MI.  Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept. 2004 Jan 15;117(1):3-10.  abstract

Tang Y, Schmitt-Ott K, Qian K, Kagiyama S, Phillips MI.  Vigilant vectors: adeno-associated virus with a biosensor to switch on amplified therapeutic genes in specific tissues in life-threatening diseases. Methods. 2002 Oct;28(2):259-66.  abstract

Tang Y, Jackson M, Qian K, Phillips MI.  Hypoxia inducible double plasmid system for myocardial ischemia gene therapy. Hypertension. 2002 Feb;39(2 Pt 2):695-8.  abstract

Phillips MI, Tang Y, Schmidt-Ott K, Qian K, Kagiyama S.  Vigilant vector: heart-specific promoter in an adeno-associated virus vector for cardioprotection. Hypertension. 2002 Feb;39(2 Pt 2):651-5.  abstract

Honors & Awards

Graduate Fellowship for Outstanding Reseach, UF College of Medicine, 2002


David G. Taylor, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Harm J. Knot, Pharm.D., Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled

August 1999 - August 2004

Dissertation Title

Roles of sarcoplasmic reticular Ca(2+) ATPase and action potential duration in normal and hypertrophied rat myocardium

Professional Positions

Postdoctoral Associate, Dept. of Pharmacology & Therapeutics, UF College of Medicine

Education

B.S., Biological Sciences, University of Southern Mississippi

Publications

Metcalfe BL, Huentelman MJ, Parilak LD, Taylor DG, Katovich MJ, Knot HJ, Sumners C, Raizada MK.  Prevention of cardiac hypertrophy by angiotensin II type-2 receptor gene transfer. Hypertension. 2004 Jun;43(6):1233-8.  abstract

Taylor DG, Parilak LD, LeWinter MM, Knot HJ. Quantification of the rat left ventricle force and Ca(2+)-frequency relationships: similarities to dog and human. Cardiovasc Res. 2004 Jan 1;61(1):77-86.  abstract

Walls SC, Taylor DG, Wilson CM.  Interspecific differences in susceptibility to competition and predation in a species-pair of larval amphibians. Herpetologica. 2002;58(1):104-118.

Abstracts Presented at Meetings Through Graduate Studies

Parilak LD, Taylor DG, Song E, Knot HJ. From hypertrophy to heart failure: Key events emerging from a parallel study in humasn and rats.  American Heart Association, 2003.

Taylor DG, Parilak LD, Knot HJ.  Quantification of calcium and force frequency in rat heart compared to humans and dogs.  FASEB 2003.

Taylor DG, Parilak LD, Knot HJ.  Effects of cardiac hypertrophy without systemic hypertension on cardiac contractility in rat and human. FASEB 2003.

Parilak LD, Taylor DG, Knot HJ.  Effects of aging and hypertension on cardiac contractility. FASEB 2003.

Honors & Awards

American Heart Association Predoctoral Fellowship, 2003-2005

Alumni Fellowship, 1999-2003

Outstanding Senior in Biology, Beta Beta Beta Biological Honor Society, University of Southern Mississippi, 2000

Honorable Mention, Graduate Research Fellowship, National Sciecne Foundation, 1999

Presidential Fellowship, University of Southern Mississippi, 1995-1999

National Merit Finalist, 1995


J. Keith Tolson, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Stephen M. Roberts, Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled

August 1997 - August 2004 (degree conferred December 2004)

Dissertation Title

Heat shock proteins and protection from hepatotoxicity

Professional Positions

Senior Toxicologist, Geosyntec

Education

B.S., Food Science, University of Florida

Publications

Halmes NC, Roberts SM, Tolson JK, Portier CJ. Reevaluating cancer risk estimates for short-term exposure scenarios. Toxicol Sci. 2000 Nov;58(1):32-42.  abstract

Tolson JK, Moye HA, Toth JP. Effect of temperature and humidity on the formation of dibutylurea in benlate fungicide. J Agric Food Chem. 1999 Mar;47(3):1217-22.  abstract

Hart CM, Tolson JK, Block ER.  Quantitative fatty acid analyses in cultured porcine pulmonary artery endothelial cells: the combined effects of fatty acid supplementation and oxidant exposure. J Cell Physiol. 1992 Oct;153(1):76-87.  abstract

Bhat GB, Tinsley SB, Tolson JK, Patel JM, Block ER.  Hypoxia increases the susceptibility of pulmonary artery endothelial cells to hydrogen peroxide injury. J Cell Physiol. 1992 May;151(2):228-38.  abstract

Hart CM, Tolson JK, Block ER.  Supplemental fatty acids alter lipid peroxidation and oxidant injury in endothelial cells. Am J Physiol. 1991 Jun;260(6 Pt 1):L481-8.  abstract

Hart CM, Tolson JK, Block ER.  Fatty acid supplementation protects pulmonary artery endothelial cells from oxidant injury. Am J Respir Cell Mol Biol. 1990 Nov;3(5):479-89.  abstract


Tiffany Wallace, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Peter P. Sayeski, Ph.D.

Department

Physiology & Functional Genomics

Enrolled

August 2001 - December 2005

Dissertation Title

Professional Positions

Education  B.S., Biology, Monmouth College

Publications

Wallace TA, Sayeski PP. Jak2 tyrosine kinase: a mediator of both housekeeping and ligand-dependent gene expression?  Cell Biochem Biophys. 2006;44(2):213-22.  abstract

Sandberg EM, Wallace TA, Godeny MD, Vonderlinden D, Sayeski PP.  Jak2 tyrosine kinase: a true jak of all trades? Cell Biochem Biophys. 2004;41(2):207-32. abstract

Wallace TA, VonDerLinden D, He K, Frank SJ, Sayeski PP.  Microarray analyses identify JAK2 tyrosine kinase as a key mediator of ligand-independent gene expression. Am J Physiol Cell Physiol. 2004 Oct;287(4):C981-91. Epub 2004 Jun 9. abstract


Fei Wang, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Stephen M. Roberts, Ph.D. 

Department

Pharmacology & Therapeutics

Enrolled

August 2001 - December 2005

Dissertation Title

Acceleration of Murine Systemic Lupus Erythematosus (SLE) by Exposure to the Organochlorine Pesticide Chlordecone

Professional Positions

Education

B.S., Science, Shanghai Jiao Tong University

Publications

Sobel ES, Wang F, Butfiloski E, Croker B, Roberts SM. Comparison of chlordecone effects on autoimmunity in (NZBxNZW) F(1) and BALB/c mice. Toxicology. 2006 Feb 1;218(2-3):81-9. Epub 2005 Nov 22.  abstract

Ketcham C, Wang F, Fisher SZ, Ercan A, Van Der Wel H, Locke RD, Ud-Doulah k S, Matta KL, West CM.  Specificity of a soluble UDP-galactose:fucoside a1,3 galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in dictyostelium. J Biol Chem. 2004 May 3 (in press)

Wang F, Metcalf T, van der Wel H, West CM.  Initiation of mucin-type O-glycosylation in dictyostelium is homologous to the corresponding step in animals and is important for spore coat function. J Biol Chem. 2003 Dec 19;278(51):51395-407.  abstract

Abstracts Presented at Meetings Through Graduate Studies

Honors & Awards

Lupus Foundation of America, Gina Finzi Memorial Student Summer Fellowship Award, 2004

American Society of Pharmacology and Experimental Therapeutics, Graduate Student Travel Award 2004


Jared T. Wilsey, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

Philip Scarpace, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1999 - August 2003

Dissertation Title

Potent anorexic and lipopenic effects of central leptin gene therapy are blocked by diet-induced obesity:  Evidence for impaired leptin receptor expression/signal transduction in obesity and reversal by caloric restriction  (full text)

Professional Positions

Research Health Scientist, VA Medical Center, Gainesville, FL

Education

B.S., Nutritional Chemistry, Cornell University, Ithaca, NY

Publications

Li G, Zhang Y, Wilsey JT, Scarpace PJ.  Unabated anorexic and enhanced thermogenic responses to melanotan II in diet-induced obese rats despite reduced melanocortin 3 and 4 receptor expression. J Endocrinol. 2004 Jul;182(1):123-32.  abstract

Wilsey J, Scarpace PJ.  Caloric restriction reverses the deficits in leptin receptor protein and leptin signaling capacity associated with diet-induced obesity: role of leptin in the regulation of hypothalamic long-form leptin receptor expression. J Endocrinol. 2004 May;181(2):297-306.  abstract

Wilsey J, Zolotukhin S, Prima V, Scarpace PJ.  Central leptin gene therapy fails to overcome leptin resistance associated with diet-induced obesity. Am J Physiol Regul Integr Comp Physiol. 2003 Nov;285(5):R1011-20.  abstract

Wilsey J, Zolotukhin S, Prima V, Shek EW, Matheny MK, Scarpace PJ.  Hypothalamic delivery of doxycycline-inducible leptin gene allows for reversible transgene expression and physiological responses. Gene Ther. 2002 Nov;9(22):1492-9.  abstract

Zhang Y, Wilsey JT, Frase CD, Matheny MM, Bender BS, Zolotukhin S, Scarpace PJ.  Peripheral but not central leptin prevents the immunosuppression associated with hypoleptinemia in rats. J Endocrinol. 2002 Sep;174(3):455-61.  abstract

Honors & Awards

University of Florida Presidential and Alumni Fellowships

Ethan Sims Young Investigator Award Finalist, North American Association for the Study of Obesity, 2001

University of Florida Medical Guild Graduate Student Research Competition, First Place, 2003


Yuan C. Zhang, Ph.D.

Advanced Concentration

Physiology & Pharmacology

Mentor

M. Ian Phillips, Ph.D.

Department

Pharmacology & Therapeutics

Enrolled

August 1996 - August 2000

Dissertation Title

A Novel B1- blocker based on antisense oligonucleotides:  A new gene therapy approach in hypertension, cardiac hypertrophy and myocardial ischemia

Professional Positions

Education

Publications

Tang YL, Tang Y, Zhang YC, Qian K, Shen L, Phillips MI.  Protection from ischemic heart injury by a vigilant heme oxygenase-1 plasmid system. Hypertension. 2004 Apr;43(4):746-51.  abstract

Tang YL, Zhao Q, Zhang YC, Cheng L, Liu M, Shi J, Yang YZ, Pan C, Ge J, Phillips MI.  Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept. 2004 Jan 15;117(1):3-10.  abstract

Kagiyama S, Eguchi S, Frank GD, Inagami T, Zhang YC, Phillips MI.  Angiotensin II-induced cardiac hypertrophy and hypertension are attenuated by epidermal growth factor receptor antisense. Circulation. 2002 Aug 20;106(8):909-12.  abstract

Chen H, Zhang YC, Li D, Phillips MI, Mehta P, Shi M, Mehta JL.  Protection against myocardial dysfunction induced by global ischemia-reperfusion by antisense-oligodeoxynucleotides directed at beta(1)-adrenoceptor mRNA. J Pharmacol Exp Ther. 2000 Aug;294(2):722-7.  abstract

Zhang YC, Bui JD, Shen L, Phillips MI.  Antisense inhibition of beta(1)-adrenergic receptor mRNA in a single dose produces a profound and prolonged reduction in high blood pressure in spontaneously hypertensive rats. Circulation. 2000 Feb 15;101(6):682-8.  abstract

Tang X, Mohuczy D, Zhang YC, Kimura B, Galli SM, Phillips MI.  Intravenous angiotensinogen antisense in AAV-based vector decreases hypertension. Am J Physiol. 1999 Dec;277(6 Pt 2):H2392-9.  abstract

Phillips MI, Zhang YC.  Basic principles of using antisense oligonucleotides in vivo. Methods Enzymol. 2000;313:46-56.  abstract

Li DY, Zhang YC, Philips MI, Sawamura T, Mehta JL.  Upregulation of endothelial receptor for oxidized low-density lipoprotein (LOX-1) in cultured human coronary artery endothelial cells by angiotensin II type 1 receptor activation. Circ Res. 1999 May 14;84(9):1043-9.  abstract

Yang BC, Phillips MI, Zhang YC, Kimura B, Shen LP, Mehta P, Mehta JL.  Critical role of AT1 receptor expression after ischemia/reperfusion in isolated rat hearts: beneficial effect of antisense oligodeoxynucleotides directed at AT1 receptor mRNA. Circ Res. 1998 Sep 7;83(5):552-9.  abstract


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last revised 11/3/06   wtm