Biochemistry & Molecular Biology

Immunology & Microbiology

Neuroscience

Genetics

Molecular Cell Biology

Physiology & Pharmacology

Bahjat K

Bahjat R

Burkhardt

Casp

Cha

Clemente

Coberley C

Gao

Gary

Gussow

He

Jurgens

Seifert K

Seifert T

Sirninger

Starks

Tran

Whittaker

Wu W

Wu Y

Zeng

Keith S. Bahjat, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Michael J. Clare-Salzler, M.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1997 - August 2001

Dissertation Title

Dendritic cell development and distribution in type I diabetes:  Overcoming genetic defects and establishing tolerance via pharmacotherapeutic manipulation of dendritic cell populations in vivo

Professional Positions

Manager of Applications Development, Cytomation, Inc., Ft. Collins, CO

Education

B.S., Oklahoma State University, Stillwater, OK

Publications

Litherland SA, She JX, Schatz D, Fuller K, Hutson AD, Peng RH, Li Y, Grebe KM, Whittaker DS, Bahjat K, Hopkins D, Fang Q, Spies PD, North K, Wasserfall C, Cook R, Dennis MA, Crockett S, Sleasman J, Kocher J, Muir A, Silverstein J, Atkinson M, Clare-Salzler MJ. Aberrant monocyte prostaglandin synthase 2 (PGS2) expression in type 1 diabetes before and after disease onset. Pediatr Diabetes. 2003 Mar;4(1):10-8.  abstract

Oberholzer A, Oberholzer C, Bahjat KS, Ungaro R, Tannahill CL, Murday M, Bahjat FR, Abouhamze Z, Tsai V, LaFace D, Hutchins B, Moldawer LL, Clare-Salzler MJ.  Increased survival in sepsis by in vivo adenovirus-induced expression of IL-10 in dendritic cells. J Immunol. 2002 Apr 1;168(7):3412-8.  abstract

Naumov YN, Bahjat KS, Gausling R, Abraham R, Exley MA, Koezuka Y, Balk SB, Strominger JL, Clare-Salzer M, Wilson SB.  Activation of CD1d-restricted T cells protects NOD mice from developing diabetes by regulating dendritic cell subsets. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13838-43.  abstract

Whittaker DS, Bahjat KS, Moldawer LL, Clare-Salzler MJ.  Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production. J Immunol. 2000 Oct 15;165(8):4298-304.  abstract

Schowengerdt KO, Fricker FJ, Bahjat KS, Kuntz ST.  Increased expression of the lymphocyte early activation marker CD69 in peripheral blood correlates with histologic evidence of cardiac allograft rejection. Transplantation. 2000 May 27;69(10):2102-7.  abstract

Ottendorfer E, Ellis TM, Bahjat KS, Clare-Salzler M, Atkinson MA.  No alteration in T lymphocyte expression of CD40 ligand (CD154) in individuals with or at increased risk for insulin-dependent diabetes mellitus. J Clin Endocrinol Metab. 1999 Nov;84(11):4068-72.  abstract

Frances Rena Bahjat, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Michael J. Clare-Salzler, M.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1998 - August 2002

Dissertation Title

Characterization and genetic analysis of the resistance of nonobese diabetic mice to tumor necrosis factor-alpha-mediated hepatocyte apoptosis and lethality

Professional Positions

Research Scientist, Nereus Pharmaceuticals, San Diego, CA

Education

B.S., Biology, Benedictine University, Lisle, IL

Publications

Blenman KR, Bahjat FR, Moldawer LL, Morel L.  Aberrant signaling in the TNFalpha/TNF receptor 1 pathway of the NZM2410 lupus-prone mouse. Clin Immunol. 2004 Feb;110(2):124-33.  abstract

Palladino MA, Bahjat FR, Theodorakis EA, Moldawer LL. Anti-TNF-alpha therapies: the next generation. Nat Rev Drug Discov. 2003 Sep;2(9):736-46.  abstract

Lam T, Ling T, Chowdhury C, Chao TH, Bahjat FR, Lloyd GK, Moldawer LL, Palladino MA, Theodorakis EA. Synthesis of a novel family of diterpenes and their evaluation as anti-inflammatory agents. Bioorg Med Chem Lett. 2003 Oct 6;13(19):3217-21.  abstract

Dharnidharka VR, Van Patten Y, Bahjat FR, Clare-Salzler M. Fas stimulation results in selective islet infiltrate apoptosis in situ and reversal of diabetes. Ann N Y Acad Sci. 2002 Apr;958:160-2.  abstract

Oberholzer A, Oberholzer C, Bahjat KS, Ungaro R, Tannahill CL, Murday M, Bahjat FR, Abouhamze Z, Tsai V, LaFace D, Hutchins B, Moldawer LL, Clare-Salzler MJ. Increased survival in sepsis by in vivo adenovirus-induced expression of IL-10 in dendritic cells. J Immunol. 2002 Apr 1;168(7):3412-8.  abstract

Oberholzer A, Oberholzer C, Bahjat FR, Edwards CK 3rd, Moldawer LL. Genetic determinants of lipopolysaccharide and D-galactosamine-mediated hepatocellular apoptosis and lethality. J Endotoxin Res. 2001;7(5):375-80.  abstract

Oberholzer C, Oberholzer A, Bahjat FR, Minter RM, Tannahill CL, Abouhamze A, LaFace D, Hutchins B, Clare-Salzler MJ, Moldawer LL. Targeted adenovirus-induced expression of IL-10 decreases thymic apoptosis and improves survival in murine sepsis. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11503-8.  abstract

Minter RM, Ferry MA, Murday ME, Tannahill CL, Bahjat FR, Oberholzer C, Oberholzer A, LaFace D, Hutchins B, Wen S, Shinoda J, Copeland EM 3rd, Moldawer LL. Adenoviral delivery of human and viral IL-10 in murine sepsis. J Immunol. 2001 Jul 15;167(2):1053-9.  abstract

Minter RM, Ferry MA, Rectenwald JE, Bahjat FR, Oberholzer A, Oberholzer C, La Face D, Tsai V, Ahmed CM, Hutchins B, Copeland EM 3rd, Ginsberg HS, Moldawer LL. Extended lung expression and increased tissue localization of viral IL-10 with adenoviral gene therapy. Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):277-82.  abstract

Bahjat FR, Dharnidharka VR, Fukuzuka K, Morel L, Crawford JM, Clare-Salzler MJ, Moldawer LL. Reduced susceptibility of nonobese diabetic mice to TNF-alpha and D-galactosamine-mediated hepatocellular apoptosis and lethality. J Immunol. 2000 Dec 1;165(11):6559-67.  abstract

Nowak M, Gaines GC, Rosenberg J, Minter R, Bahjat FR, Rectenwald J, MacKay SL, Edwards CK 3rd, Moldawer LL. LPS-induced liver injury in D-galactosamine-sensitized mice requires secreted TNF-alpha and the TNF-p55 receptor. Am J Physiol Regul Integr Comp Physiol. 2000 May;278(5):R1202-9.  abstract

Josephs MD, Bahjat FR, Fukuzuka K, Ksontini R, Solorzano CC, Edwards CK 3rd, Tannahill CL, MacKay SL, Copeland EM 3rd, Moldawer LL. Lipopolysaccharide and D-galactosamine-induced hepatic injury is mediated by TNF-alpha and not by Fas ligand. Am J Physiol Regul Integr Comp Physiol. 2000 May;278(5):R1196-201.  abstract

Honors & Awards

T32 NIH Training Grant Pre-doctoral Fellowship (2000-2001)

Freshman Chemistry Award from Indiana University (1994)

Award for Excellence in the field of Biology from Benedictine University (all semesters)

St. Benedict Award and scholarship for academic achievements (all semesters)

Benedictine University Dean’s list (all semesters)

Kim Blenman, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Laurence Morel, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled 

August 2000 - April 2005

Education

B.S., Chemistry, University of Florida

Dissertation Research

Apoptosis and inflammation in a mouse model of systemic lupus erythematosus

Publications

Morel L, Blenman KR, Croker BP, Wakeland EK. The major murine systemic lupus erythematosus susceptibility locus, Sle1, is a cluster of functionally related genes. Proc Natl Acad Sci USA. 2001 Feb 13;98(4):1787-92.  abstract

Morel L, Croker BP, Blenman KR, Mohan C, Huang G, Gilkeson G, Wakeland EK. Genetic reconstitution of systemic lupus erythematosus immunopathology with polycongenic murine strains. Proc Natl Acad Sci USA. 2000 Jun 6;97(12):6670-5.  abstract

Morel L, Yu Y, Blenman KR, Caldwell RA, Wakeland EK. Production of congenic mouse strains carrying genomic intervals containing SLE-susceptibility genes derived from the SLE-prone NZM2410 strain. Mamm Genome. 1996 May;7(5):335-9.  abstract

Honors & Awards

University Women's Club Scholarship for Graduate Students, 2004

Todd M. Brusko, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Mark Atkinson, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 2002 - August 2006

Dissertation Title

Professional Positions

Education

B.S., Microbiology & Cell Science, University of Florida

Publications

Araya CE, Wasserfall CH, Brusko TM, Mu W, Segal MS, Johnson RJ, Garin EH. A case of unfulfilled expectations. Cytokines in idiopathic minimal lesion nephrotic syndrome. Pediatr Nephrol. 2006 Mar 7; [Epub ahead of print] abstract

Burkhardt BR, Greene SR, White P, Wong RK, Brestelli JE, Yang J, Robert CE, Brusko TM, Wasserfall CH, Wu J, Atkinson MA, Gao Z, Kaestner KH, Wolf BA.  PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21). Gene. 2006 Mar 15;369C:134-141.  abstract

Brusko TM, Wasserfall CH, Agarwal A, Kapturczak MH, Atkinson MA.  An Integral Role for Heme Oxygenase-1 and Carbon Monoxide in Maintaining Peripheral Tolerance by CD4⁺CD25⁺ Regulatory T Cells. J Immunol. 2005 May 1;174(9):5181-6.  abstract

Brusko TM, Wasserfall CH, Clare-Salzler MJ, Schatz DA, Atkinson MA.  Functional Defects and the Influence of Age on the Frequency of CD4⁺CD25⁺ T-Cells in Type 1 Diabetes. Diabetes. 2005 May;54(5):1407-14.  abstract

You S, Chen C, Lee WH, Brusko T, Atkinson M, Liu CP.  Presence of diabetes-inhibiting, glutamic acid decarboxylase-specific, IL-10-dependent, regulatory T cells in naive nonobese diabetic mice. J Immunol. 2004 Dec 1;173(11):6777-85.  abstract

Haller MJ, Samyn M, Nichols WW, Brusko T, Wasserfall C, Schwartz RF, Atkinson M, Shuster JJ, Pierce GL, Silverstein JH.  Radial artery tonometry demonstrates arterial stiffness in children with type 1 diabetes. Diabetes Care. 2004 Dec;27(12):2911-7.  abstract

Kapturczak MH, Wasserfall C, Brusko T, Campbell-Thompson M, Ellis TM, Atkinson MA, Agarwal A. Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse. Am J Pathol. 2004 Sep;165(3):1045-53.  abstract

Morales A, Wasserfall C, Brusko T, Carter C, Schatz D, Silverstein J, Ellis T, Atkinson M.  Adiponectin and leptin concentrations may aid in discriminating disease forms in children and adolescents with type 1 and type 2 diabetes. Diabetes Care. 2004 Aug;27(8):2010-4.  abstract

Guo D, Li M, Zhang Y, Yang P, Eckenrode S, Hopkins D, Zheng W, Purohit S, Podolsky RH, Muir A, Wang J, Dong Z, Brusko T, Atkinson M, Pozzilli P, Zeidler A, Raffel LJ, Jacob CO, Park Y, Serrano-Rios M, Larrad MT, Zhang Z, Garchon HJ, Bach JF, Rotter JI, She JX, Wang CY.  A functional variant of SUMO4, a new IkappaBalpha modifier, is associated with type 1 diabetes. Nat Genet. 2004 Aug;36(8):837-41.  abstract

Zhang YC, Powers M, Wasserfall C, Brusko T, Song S, Flotte T, Snyder RO, Potter M, Scott-Jorgensen M, Campbell-Thompson M, Crawford JM, Nick HS, Agarwal A, Ellis TM, Atkinson MA. Immunity to adeno-associated virus serotype 2 delivered transgenes imparted by genetic predisposition to autoimmunity. Gene Ther. 2004 Feb;11(3):233-40.  abstract

Goudy KS, Burkhardt BR, Wasserfall C, Song S, Campbell-Thompson ML, Brusko T, Powers MA, Clare-Salzler MJ, Sobel ES, Ellis TM, Flotte TR, Atkinson MA. Systemic overexpression of IL-10 induces CD4+CD25+ cell populations in vivo and ameliorates type 1 diabetes in nonobese diabetic mice in a dose-dependent fashion. J Immunol. 2003 Sep 1;171(5):2270-8.  abstract

Zhang YC, Pileggi A, Agarwal A, Molano RD, Powers M, Brusko T, Wasserfall C, Goudy K, Zahr E, Poggioli R, Scott-Jorgensen M, Campbell-Thompson M, Crawford JM, Nick H, Flotte T, Ellis TM, Ricordi C, Inverardi L, Atkinson MA. Adeno-associated virus-mediated IL-10 gene therapy inhibits diabetes recurrence in syngeneic islet cell transplantation of NOD mice. Diabetes. 2003 Mar;52(3):708-16.  abstract

Goudy K, Wasserfall C, Burkhardt BA, Brusko T, Song S, Ellis T, Flotte T, Atkinson M.  Evaluation of mechanism, dose, and time dependency for AAV-IL-10 gene therapy in NOD mice.  Diabetes.  2002 Jun;51:1159 Suppl. 2.

Abstracts Presented at Meetings Through Graduate Studies

Wasserfall C, Schwartz RF, Simon G, Binns SA, Brusko T, Stalvey M, Clare-Salzler M, Song S, Campbell-Thompson M, Flotte T, Atkinson M.  A dichotomous role for interleukin-10 in the pathogenesis of type 1 diabetes.  DIABETES 54: A24-A24 Suppl. 1 2005

Tang M, Lu Y, Brusko T, Wasserfall C, Zhang B, Campbell-Thompson M, Atkinson MA, Song S.  Prevention of type 1 diabetes by AAT gene therapy is dose and time dependent.  DIABETES 54: A312-A313 Suppl. 1, 2005

Haller M, Cooper S, Putnam A, Freed B, Brusko T, Chase P, Atkinson M, Schatz D.  Autologous cord blood transfusion associated with prolonged honeymoon in a child with type 1 diabetes (T1D). DIABETES 54: A485-A485 Suppl. 1, 2005

Stalvey M, Wasserfall C, Brusko T, Flotte T, Schatz D, Atkinson M.  A pro-inflammatory state and hyperglycemia are associated with a cystic fibrosis related diabetes mouse model.  DIABETES 54: A645-A645 Suppl. 1, 2005

Todd M. Brusko, Clive H. Wasserfall, Michael J. Clare-Salzler, Desmond A. Schatz, Mark A. Atkinson.  65th Scientific Sessions; Meeting of the American Diabetes Association, 2005.  "Functional Defects and the Influence of Age on the Frequency of CD4+CD25+ T Cells in Type 1 Diabetes", Abstract Number: 246-OR.  

Todd M. Brusko, Clive H. Wasserfall, Schatz, Mark A. Atkinson.  65th Scientific Sessions; Meeting of the American Diabetes Association, 2005.  "Dynamics of CD4+CD25+ T Cells and Soluble CD25 in Type 1 Diabetes", Abstract Number: 248-OR.

Honors & Awards

2005 Graduate Fellowship for Outstanding Research, UF College of Medicine

Brant R. Burkhardt, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Maureen M. Goodenow, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1996 - December 2001

Dissertation Title

Phylogenetic and promoter analysis of the human immodeficiency virus type 1 (hiv-1) long terminal repeat and envelope regions from the tissues of vertically infected patients

Professional Positions

Postdoctoral Associate, Dept. Pathology, Immunology & Laboratory Medicine (Mark Atkinson, Ph.D.), UF College of Medicine

Education

B.S., New Jersey Institute of Technology, Newark, NJ

Ed.M.S., Clemson University, Clemson, SC

Publications

Burkhardt BR, Greene SR, White P, Wong RK, Brestelli JE, Yang J, Robert CE, Brusko TM, Wasserfall CH, Wu J, Atkinson MA, Gao Z, Kaestner KH, Wolf BA.  PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21). Gene. 2006 Mar 15;369C:134-141.  abstract

Burkhardt BR, Parker MJ, Zhang YC, Song S, Wasserfall CH, Atkinson MA. Glucose transporter-2 (GLUT2) promoter mediated transgenic insulin production reduces hyperglycemia in diabetic mice. FEBS Lett. 2005 Oct 24;579(25):5759-64.  abstract

Burkhardt BR, Loiler SA, Anderson JA, Kilberg MS, Crawford JM, Flotte TR, Goudy KS, Ellis TM, Atkinson M. Glucose-responsive expression of the human insulin promoter in HepG2 human hepatoma cells. Ann N Y Acad Sci. 2003 Nov;1005:237-41.  abstract

Goudy KS, Burkhardt BR, Wasserfall C, Song S, Campbell-Thompson ML, Brusko T, Powers MA, Clare-Salzler MJ, Sobel ES, Ellis TM, Flotte TR, Atkinson MA. Systemic overexpression of IL-10 induces CD4+CD25+ cell populations in vivo and ameliorates type 1 diabetes in nonobese diabetic mice in a dose-dependent fashion. J Immunol. 2003 Sep 1;171(5):2270-8.  abstract

Perez EE, Rose SL, Peyser B, Lamers SL, Burkhardt B, Dunn BM, Hutson AD, Sleasman JW, Goodenow MM. Human immunodeficiency virus type 1 protease genotype predicts immune and viral responses to combination therapy with protease inhibitors (PIs) in PI-naive patients. J Infect Dis. 2001 Feb 15;183(4):579-88.  abstract

Kouba AJ, Burkhardt BR, Alvarez IM, Goodenow MM, Buhi WC. Oviductal plasminogen activator inhibitor-1 (PAI-1): mRNA, protein, and hormonal regulation during the estrous cycle and early pregnancy in the pig. Mol Reprod Dev. 2000 Jul;56(3):378-86.  abstract

Courtney B. Casp, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Wayne T. McCormack, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1998 - December 2003

Dissertation Title

Genetic association of catalase and antigen processing genes with vitiligo susceptibility   full text

Professional Positions

Law Student, Levin College of Law, University of Florida

Education

B.S., Biology, University of Richmond

Publications

Casp CB, She JX, McCormack WT.  Genes of the LMP/TAP cluster are associated with the human autoimmune disease vitiligo. Genes Immun. 2003 Oct;4(7):492-9.  abstract

Casp CB, She JX, McCormack WT.  Genetic association of the catalase gene (CAT) with vitiligo susceptibility. Pigment Cell Res. 2002 Feb;15(1):62-6.   abstract

Abstracts Presented at Meetings Through Graduate Studies

Kristensen, D., C.A. Bradley, M. Hao, S. Perkins, W.T. McCormack. 2000. Case/control association study of candidate genes for vitiligo susceptibility: tyrosine metabolism and immune response genes. 52nd Annual Meeting & Clin. Lab. Expo., Amer. Acad. Clin. Chem.

Bradley, C.A., D. Kristensen, M. Hao, S. Perkins, M. Marron, J.X. She, W.T. McCormack. Case/control association study of immune response genes and vitiligo susceptibility. 2000. FASEB J. 14(6):A1109.

Bradley, C.A., D. Kristensen, J.X. She, W.T. McCormack. 2001. Genetic association studies of immune response genes and vitiligo susceptibility. FASEB J. 15(4):A323.

McCormack, W.T., Bradley, C.A., D. Kristensen, J.X. She. 2001. Genetics of vitiligo susceptibility: Association studies of immune response genes. Pigment Cell Res. 14(3):231.

Casp, C.B., S.A. Litherland, J.-X. She, W.T. McCormack. 2002. Antigen processing and presentation genes in vitiligo susceptibility. FASEB J. 16(5):A1219.

Seunghee Cha, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Michael Humphreys-Beher, Ph.D.

Department

Oral Biology

Enrolled

August 1996 - December 2001

Dissertation Title

Abnormal organogenesis in salivary gland development may initiate adult onset of autoimmune exocrinopathy

Professional Positions

Postdoctoral Associate, Dept. of Oral Biology, University of Florida College of Dentistry

Education

D.D.S., Yonsei University, Seoul, Korea

Publications

Gao J, Killedar S, Cornelius JG, Nguyen C, Cha S, Peck AB. Sjogren's syndrome in the NOD mouse model is an interleukin-4 time-dependent, antibody isotype-specific autoimmune disease. J Autoimmun. 2006 Mar;26(2):90-103.  abstract

Gao J, Cha S, Jonsson R, Opalko J, Peck AB. Detection of anti-type 3 muscarinic acetylcholine receptor autoantibodies in the sera of Sjogren's syndrome patients by use of a transfected cell line assay. Arthritis Rheum. 2004 Aug;50(8):2615-21. abstract

Cha S, Nagashima H, Peck AB, Humphreys-Beher MG.  IDD3 and IDD5 alleles from nod mice mediate Sjogren's syndrome-like autoimmunity. Adv Exp Med Biol. 2002;506(Pt B):1035-9.  abstract

Humphreys-Beher MG, Brayer J, Cha S, Nagashima H, Diggs S, Peck AB.  Immunogenetics of autoimmune exocrinopathy in the nod mouse: more than meets the eye. Adv Exp Med Biol. 2002;506(Pt B):999-1007.  abstract

Cha S, Nagashima H, Brown VB, Peck AB, Humphreys-Beher MG.  Two NOD Idd-associated intervals contribute synergistically to the development of autoimmune exocrinopathy (Sjogren's syndrome) on a healthy murine background. Arthritis Rheum. 2002 May;46(5):1390-8.  abstract

Cha S, Peck AB, Humphreys-Beher MG.  Progress in understanding autoimmune exocrinopathy using the non-obese diabetic mouse: an update. Crit Rev Oral Biol Med. 2002;13(1):5-16.  abstract

Cha S, van Blockland SC, Versnel MA, Homo-Delarche F, Nagashima H, Brayer J, Peck AB, Humphreys-Beher MG.  Abnormal organogenesis in salivary gland development may initiate adult onset of autoimmune exocrinopathy. Exp Clin Immunogenet. 2001;18(3):143-60.  abstract

Nagy A, Nagashima H, Cha S, Oxford GE, Zelles T, Peck AB, Humphreys-Beher MG.  Reduced oral wound healing in the NOD mouse model for type 1 autoimmune diabetes and its reversal by epidermal growth factor supplementation. Diabetes. 2001 Sep;50(9):2100-4.  abstract

Brayer JB, Cha S, Nagashima H, Yasunari U, Lindberg A, Diggs S, Martinez J, Goa J, Humphreys-Beher MG, Peck AB.  IL-4-dependent effector phase in autoimmune exocrinopathy as defined by the NOD.IL-4-gene knockout mouse model of Sjogren's syndrome. Scand J Immunol. 2001 Jul-Aug;54(1-2):133-40.  abstract

Clarke MY, Brayer J, Heintz K, Nagashima H, Cha S, Oxford GE, Nanni JM, Peck AB, Zelles T, Humphreys-Beher MG.  Differential absorption and distribution of epidermal growth factor and insulin-like growth factor in diabetic NOD mice. J Diabetes Complications. 2001 Mar-Apr;15(2):103-11.  abstract

Nguyen KH, Brayer J, Cha S, Diggs S, Yasunari U, Hilal G, Peck AB, Humphreys-Beher MG.  Evidence for antimuscarinic acetylcholine receptor antibody-mediated secretory dysfunction in nod mice. Arthritis Rheum. 2000 Oct;43(10):2297-306.  abstract

Brayer J, Lowry J, Cha S, Robinson CP, Yamachika S, Peck AB, Humphreys-Beher MG.  Alleles from chromosomes 1 and 3 of NOD mice combine to influence Sjogren's syndrome-like autoimmune exocrinopathy. J Rheumatol. 2000 Aug;27(8):1896-904.   abstract

Honors & Awards

Maui 2000 Conference Grant, Third International Conference on the Lacrimal Gland, Tear Film and Dry Eye Syndromes

Mary Ann Checkley, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Alfred S. Lewin, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled 

August 1998 - April 2005

Dissertation Research

Identification and characterization of potential virulence factors expressed by Candida albicans during oropharyngeal candidiasis in HIV-infected patients

Publications

Cheng S, Clancy CJ, Checkley MA, Handfield M, Hillman JD, Progulske-Fox A, Lewin AS, Fidel PL, Nguyen MH. Identification of Candida albicans genes induced during thrush offers insight into pathogenesis. Mol Microbiol. 2003 Jun;48(5):1275-88.  abstract

Jose C. Clemente, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Ben M. Dunn, Ph.D.

Department

Biochemistry & Molecular Biology

Enrolled

August 1998 - May 2003

Dissertation Title

Analysis of therapy selected non-active site mutations in the HIV-1 protease

Professional Positions

Postdoctoral Associate, Dept. Biochemistry & Molecular Biology, UF College of Medicine

Education

B.S., University of Florida

Publications

Clemente JC, Hemrajani R, Blum LE, Goodenow MM, Dunn BM.  Secondary Mutations M36I and A71V in the Human Immunodeficiency Virus Type 1 Protease Can Provide an Advantage for the Emergence of the Primary Mutation D30N. Biochemistry. 2003 Dec 30;42(51):15029-35.  abstract

Wlodawer A, Li M, Gustchina A, Oyama H, Oda K, Beyer BB, Clemente J, Dunn BM.  Two inhibitor molecules bound in the active site of Pseudomonas sedolisin: a model for the bi-product complex following cleavage of a peptide substrate. Biochem Biophys Res Commun. 2004 Feb 6;314(2):638-45.  abstract

Carter R. Coberley, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Maureen M. Goodenow, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1997 - August 2002

Dissertation Title

Phenotypic and functional genomic characterization of immune-cell reservoirs for Human Immunodeficiency Virus Type-1  (full text)

Professional Positions

Bioinformatics Research Analyst II, Gene Logic Inc., Gaithersburg, MD

Education

B.S., Microbiology, Oregon State University, Corvallis, OR

Publications

Kohler JJ, Tuttle DL, Coberley CR, Sleasman JW, Goodenow MM.  Human immunodeficiency virus type 1 (HIV-1) induces activation of multiple STATs in CD4+ cells of lymphocyte or monocyte/macrophage lineages. J Leukoc Biol. 73(3):407-16. 2003.  abstract

Honors & Awards

Waldo-Cummings Outstanding Student Award, Oregon State University, 1993

Microbiology Students Association Vice President, Oregon State University, 1993-1994

Phi Kappa Phi National Honor Society, Oregon State University, 1993-1994

NIH Rheumatology Training Grant Awardee, UF College of Medicine, 2001

Sadie S. Coberley, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Paul A. Klein, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1997 - August 2002

Dissertation Title

The role of herpesviruses in marine turtle diseases  (full text)

Professional Positions

Research Associate, US Army Medical Research Institute of Infectious Diseases, Virology Division, Fort Detrick, MD

Education

Publications

Coberley SS, Condit RC, Herbst LH, Klein PA.  Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus. J Virol. 2002 Oct;76(20):10553-8.  abstract

Coberley SS, Herbst LH, Ehrhart LM, Bagley DA, Hirama S, Jacobson ER, Klein PA.  Survey of Florida green turtles for exposure to a disease-associated herpesvirus. Dis Aquat Organ. 2001 Dec 5;47(3):159-67.  abstract

Coberley SS, Herbst LH, Brown DR, Ehrhart LM, Bagley DA, Schaf SA, Moretti RH, Jacobson ER, Klein PA.  Detection of antibodies to a disease-associated herpesvirus of the green turtle, Chelonia mydas. J Clin Microbiol. 2001 Oct;39(10):3572-7.  abstract

Honors & Awards

National Academies Research Associate Award, "Use of Filovirus Specific Antibodies to Evaluate Mechanisms of Virus Neutralization and Protective Epitopes", 2002

Yiqian Dong, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Robert Burne, Ph.D. 

Department

Oral Biology

Enrolled

August 2001 - December 2004

Dissertation Title

The arginine deiminase system of Streptococcus gordonii DL-1

Professional Positions

Education

Publications

Dong Y, Chen YY, Burne RA.  Control of expression of the arginine deiminase operon of Streptococcus gordonii by CcpA and Flp. J Bacteriol. 2004 Apr;186(8):2511-4.  abstract

Dong Y, Chen YY, Snyder JA, Burne RA.  Isolation and molecular analysis of the gene cluster for the arginine deiminase system from Streptococcus gordonii DL1. Appl Environ Microbiol. 2002 Nov;68(11):5549-53.  abstract

Brian R. Dorn, Ph.D., J.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Ann Progulske-Fox, Ph.D.

Department

Oral Biology

Enrolled

August 1997 - May 2003

Dissertation Title

Interactions between Porphyromonas gingivalis and endothelial cells

Professional Positions

Associate Attorney, Merchant and Gould, Minneapolis, MN

Education

B.S., Microbiology, University of Michigan, Ann Arbor, MI

M.S., Oral Biology, UF College of Dentistry  

J.D., UF Levin College of Law

Publications

Dorn BR, Harris LJ, Wujick CT, Vertucci FJ, Progulske-Fox A.  Invasion of vascular cells in vitro by Porphyromonas endodontalis. Int Endod J. 2002 Apr;35(4):366-71.  abstract

Dorn BR, Dunn WA Jr, Progulske-Fox A.  Bacterial interactions with the autophagic pathway. Cell Microbiol. 2002 Jan;4(1):1-10.  abstract

Dorn BR, Dunn WA Jr, Progulske-Fox A.  Porphyromonas gingivalis traffics to autophagosomes in human coronary artery endothelial cells. Infect Immun. 2001 Sep;69(9):5698-708.  abstract

Dorn BR, Burks JN, Seifert KN, Progulske-Fox A.  Invasion of endothelial and epithelial cells by strains of Porphyromonas gingivalis. FEMS Microbiol Lett. 2000 Jun 15;187(2):139-44.  abstract

Progulske-Fox A, Kozarov E, Dorn B, Dunn W Jr, Burks J, Wu Y.  Porphyromonas gingivalis virulence factors and invasion of cells of the cardiovascular system. J Periodontal Res. 1999 Oct;34(7):393-9.  abstract

Dorn BR, Dunn WA Jr, Progulske-Fox A.  Invasion of human coronary artery cells by periodontal pathogens. Infect Immun. 1999 Nov;67(11):5792-8.  abstract

Dorn BR, Leung KL, Progulske-Fox A.  Invasion of human oral epithelial cells by Prevotella intermedia. Infect Immun. 1998 Dec;66(12):6054-7.  abstract

Honors & Awards

Travel Grants, American Society for Microbiology, 1998, 1999

Travel Grants, University of Florida Graduate Student Council, 1998, 1999, 2000

Finalist, American Association for Dental Research/Warner-Lambert Hatton Competition, American Assoc. for Dental Research, 2000

Finalist, International Association for Dental Research/Warner-Lambert Hatton Competition, International Assoc. for Dental Research, 2000

First Place, Immunology & Microbiology, Medical Guild Graduate Student Research Competition, UF College of Medicine, 2000

Biyan Duan, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Laurence Morel, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 2000 - May 2006

Dissertation Title

Characterization of the B cells in lupus-prone and resistant mouse models

Professional Positions

Education

Publications

Xu Z, Duan B, Croker BP, Wakeland EK, Morel L. Genetic dissection of the murine lupus susceptibility locus Sle2: contributions to increased peritoneal B-1a cells and lupus nephritis map to different loci. J Immunol. 2005 Jul 15;175(2):936-43. abstract

Erickson LD, Lin LL, Duan B, Morel L, Noelle RJ. A genetic lesion that arrests plasma cell homing to the bone marrow. Proc Natl Acad Sci USA. 2003 Oct 28;100(22):12905-10.  abstract

Juehua Gao, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Ammon B. Peck, Ph.D. 

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1999 - December 2004

Dissertation Title

Biological functions and molecular mechanisms of the interleukin 4 signaling pathways in autoimmune exocrinopathy using the NOD.B10-H2b mouse model of Sjögren's Syndrome

Professional Positions

Education

Publications

Gao J, Killedar S, Cornelius JG, Nguyen C, Cha S, Peck AB. Sjogren's syndrome in the NOD mouse model is an interleukin-4 time-dependent, antibody isotype-specific autoimmune disease. J Autoimmun. 2006 Mar;26(2):90-103.  abstract

Gao J, Cha S, Jonsson R, Opalko J, Peck AB.  Detection of anti-type 3 muscarinic acetylcholine receptor autoantibodies in the sera of Sjogren's syndrome patients by use of a transfected cell line assay. Arthritis Rheum. 2004 Aug;50(8):2615-21.  abstract

Lee William Gary, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

David C. Bloom, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - May 2004

Dissertation Title

Identification of a virulence function in Herpes simplex virus type I mapping to the latency-associated transcript locus

Professional Positions

Postdoctoral Fellow, Department of Neurology, University of Colorado, Denver, CO

Education

B.S., Mars Hill College, Mars Hill, NC

Publications

Sun A, Devi-Rao GV, Rice MK, Gary LW, Bloom DC, Sandri-Goldin RM, Ghazal P, Wagner EK.  Immediate-early expression of the herpes simplex virus type 1 ICP27 transcript is not critical for efficient replication in vitro or in vivo. J Virol. 2004 Oct;78(19):10470-8.  abstract

Ann Griswold, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Department

Enrolled 

August 2001 - May 2006

Dissertation Title

Ammonia-generating mechanisms of mutans streptococci

Professional Positions

Education

B.S.,  Microbiology,  University of Maryland at College Park

Publications

Griswold AR, Jameson-Lee M, Burne RA. Regulation and physiologic significance of the agmatine deiminase system of Streptococcus mutans UA159.  J Bacteriol. 2006 Feb;188(3):834-41. abstract

Griswold A, Chen YY, Snyder JA, Burne RA.  Characterization of the arginine deiminase operon of Streptococcus rattus FA-1. Appl Environ Microbiol. 2004 Mar;70(3):1321-7. abstract

Griswold AR, Chen YY, Burne RA.  Analysis of an agmatine deiminase gene cluster in Streptococcus mutans UA159. J Bacteriol. 2004 Mar;186(6):1902-4. abstract

First Place, Immunology & Microbiology Advanced Concentration, Medical Guild Graduate Student Research Concentration, 2005

Bronze Medal Finalist, Medical Guild Graduate Student Research Competition, 2005

Anne Gussow, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

David C. Bloom, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

January 2000 - May 2004

Dissertation Title

Characterization of a transgenic mouse expressing the Herpes simplex virus type 1 latency-associated transcript

Professional Positions

Postdoctoral Associate, National Cancer Institute, National Institutes of Health, Bethesda, MD

Education

B.S., Susquehanna University, Selinsgrove, PA

Unhwan Ha, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Shouguang Jin, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - August 2002

Dissertation Title

Pathogenicity of Pseudomonas aeruginosa:  Bacterial invasion, intracellular survival and in vivo gene expression

Professional Positions

Postdoctoral Associate, Molecular Genetics & Microbiology (Shouguang Jin, Ph.D.), UF College of Medicine

Postdoctoral Fellow, House Ear Institute, Section on Signal Transduction, Los Angeles, CA

Education

B.S., Engineering, Korea University

M.S., Korea University

Publications

Ha UH, Wang Y, Jin S.  DsbA of Pseudomonas aeruginosa is essential for multiple virulence factors. Infect Immun. 2003 Mar;71(3):1590-5.  abstract

Wang Y, Ha U, Zeng L, Jin S.  Regulation of membrane permeability by a two-component regulatory system in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2003 Jan;47(1):95-101.  abstract

Ha U, Jin S.  Growth phase-dependent invasion of Pseudomonas aeruginosa and its survival within HeLa cells. Infect Immun. 2001 Jul;69(7):4398-406.  abstract

Kaufman MR, Jia J, Zeng L, Ha U, Chow M, Jin S.  Pseudomonas aeruginosa mediated apoptosis requires the ADP-ribosylating activity of exoS. Microbiology. 2000 Oct;146 ( Pt 10):2531-41.  abstract

Ha U, Jin S.  Expression of the soxR gene of Pseudomonas aeruginosa is inducible during infection of burn wounds in mice and is required to cause efficient bacteremia. Infect Immun. 1999 Oct;67(10):5324-31.  abstract

Jin He, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Lung-Ji Chang, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - August 2004

Dissertation Title

Lentiviral vectors: Mechanisms of transgene silencing and functional characterization of novel genes

Professional Positions

Postdoctoral Associate, Dept. Molecular Genetics & Microbiology, UF College of Medicine

Education

B.S., Clinical Medicine, Zhejiang Medical University (P.R. China)

Publications

Chang LJ, Liu X, He J. Lentiviral siRNAs targeting multiple highly conserved RNA sequences of human immunodeficiency virus type 1. Gene Therapy. (in press)

He J, Chang LJ.  Functional characterization of hepatoma-specific stem cell antigen-2. Mol Carcinog. 2004 Jun;40(2):90-103.  abstract

Chang LJ, He J. Retroviral vectors for gene therapy of AIDS and cancer. Curr Opin Mol Ther. 2001 Oct;3(5):468-75.  abstract

Abstracts Presented at Meetings Through Graduate Studies

Chen XC, He J, Chang LJ. Improved dendritic cell modality using lentiviral vector. The 95th Annual Meeting of the American Associate for Cancer Research, Orlando, FL, March27-31, 2004.

He J, Chang LJ. Long-term silencing of tumor specific gene by lentiviral vectors expressing small interference RNA. The 6th Annual Meeting of the American Society of Gene Therapy. Washington, DC, June 4-8, 2003.

He J, Chang LJ. Identification of highly expressed tumor specific genes by a suppression subtractive hybridization method and characterization of gene functions using small interference RNA. The 10th Annual Meeting of the European Society of Gene Therapy, Antibes, France, October 13-16, 2002.

Higashikawa F, He J, Chang LJ. Gene expression in oncoretrovial and lentiviral transduced human HUVC. The 4th Annual Meeting of the American Society of Gene Therapy. Seattle, WA, 2001.

Honors & Awards

Medical Guild Research Day, College of Medicine, University of Florida, First Place Immunology & Microbiology, Fifth place Overall, 2003

David E. Holmes, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Sue A. Moyer, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1996 - August 2001

Dissertation Title

Identification of the phosphoprotein binding site on the L polymerase subunit of negative strand RNA viruses

Professional Positions

Postdoctoral Associate, U.S. Dept. of Agriculture, Agicultural Research Service, Arthropod-Borne Animal Diseases Research Laboratory (ABADRL), Laramie, WY

Education

B.S., University of Florida

Publications

Cevik B, Holmes DE, Vrotsos E, Feller JA, Smallwood S, Moyer SA.  The phosphoprotein (P) and L binding sites reside in the N-terminus of the L subunit of the measles virus RNA polymerase. Virology. 2004 Oct 1;327(2):297-306.  abstract

Holmes DE, Moyer SA.  The phosphoprotein (P) binding site resides in the N terminus of the L polymerase subunit of sendai virus. J Virol. 2002 Mar;76(6):3078-83.  abstract

Jinghua Jia, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Shouguang Jin, Ph.D. 

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - August 2004

Dissertation Title

Pseudomonas aeruginosa ExoS induced apoptosis in host cells

Professional Positions

Postdoctoral Associate, Dept. Molecular Genetics & Microbiology, UF College of Medicine

Education

B.S., Biochemistry, Nankai University;  M.S., Biochemistry, Nankai University School of Life Sciences (P.R. China)

Publications

Jia J, Jin S.  Pseudomonas aeruginosa ExoS is sufficient to induce apoptosis in HeLa cells.  (in preparation)

Jia J, Ha U, Kim J, Wu D, Jin S.  Copper stress effectively inhibits the type III secretion system of Pseudomonas aeruginosa through PtrA.  (in preparation)

Ha U, Kim J, Badrane H, Jia J, Baker HV, Wu D, Jin S.  An in vivo inducible gene of Pseudomonas aeruginosa encodes a repressor for the type III secretion system.  Submitted to Molecular Microbiology

Ahn KS, Ha U, Jia J, Wu D, Jin S.  The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes. Microbiology. 2004 Mar;150(Pt 3):539-47.   abstract

Jia J, Alaoui-El-Azher M, Chow M, Chambers TC, Baker H, Jin S.  c-Jun NH2-terminal kinase-mediated signaling is essential for Pseudomonas aeruginosa ExoS-induced apoptosis. Infect Immun. 2003 Jun;71(6):3361-70.   abstract

Kaufman MR, Jia J, Zeng L, Ha U, Chow M, Jin S.  Pseudomonas aeruginosa mediated apoptosis requires the ADP-ribosylating activity of exoS. Microbiology. 2000 Oct;146 (Pt 10):2531-41.   abstract

Christy K. Jurgens, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

James B. Flanegan, Ph.D.

Department

Biochemistry & Molecular Biology

Enrolled

August 1997 - May 2002

Dissertation Title

Role of poliovirus P2 proteins in regulating the stability, translation, and replication of viral genomic RNA

Professional Positions

Postdoctoral Associate, Dept. Microbiology & Immunology, Univ. North Carolina, Chapel Hill, NC

Postdoctoral Research Associate, Carolina Vaccine Institute, Univ. North Carolina, Chapel Hill, NC

Education

B.S., University of Georgia, Athens, GA

Publications

Jurgens C, Flanegan JB.  Initiation of poliovirus negative-strand RNA synthesis requires precursor forms of p2 proteins. J Virol. 2003 Jan;77(2):1075-83.  abstract

Sarah Koch, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Maureen M. Goodenow, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled 

August 2001 - December 2005

Dissertation Title

Dominant effect of Human Immunodeficiency Virus Type 1 (HIV-1) gag-pol on viral fitness and resistance to protease inhibitors

Dominant effect of Human Immunodeficiency Virus Type 1 (HIV-1) gag-pol on viral fitness and resistance to protease inhibitors

Professional Positions 

Education

B.S., Biology, Emory University

Koch SJ, Rose SL, Pomeroy SM, O'Brien P, Dunn BM, Sleasman JW, Goodenow MM.  Polymorphisms in HIV-1 gag-pol Impact Fitness in a Cell-Type Specific Manner.  11th Conference on Retroviruses and Oppurtunistis Infections.  February 8-11, 2004.  San Francisco, California.

Rose SL, Koch SJ, Pomeroy SM, Lamers SL, Sleasman JW, Goodenow MM.  Segregation of HIV-1 protease genotypic resistance within blood compartments identifies the CD4+ CD45RA T lymphocytes as a long-loved viral reservoir.  1st International Workshop on HIV Persistence during Therapy.  December 10-12, 2003.  Saint Martin, French West Indies

Nicole Kubat, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

David C. Bloom, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - May 2004

Dissertation Title

Differential association of acetyl-histone H3 (K9, K14) with specific regions of the HSV-1 genome during latency and reactivation in the mouse model  

Professional Positions

Postdoctoral Associate, California Institute of Technology

Education

B.S., Nebraska Wesleyan University, Lincoln, NE

Publications

Kubat NJ, Tran RK, McAnany P, Bloom DC.  Specific histone tail modification and not DNA methylation is a determinant of herpes simplex virus type 1 latent gene expression. J Virol. 2004 Feb;78(3):1139-49.  abstract

Amy MacNeill, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Richard W. Moyer, Ph.D. 

Department

Enrolled 

Dissertation Title

Alterations of poxvirus serpins have unexpected effects on virus pathogenesis

Professional Positions  

Education

B.S., Chemistry, University of Florida

Publications

Nathaniel R, MacNeill AL, Wang YX, Turner PC, Moyer RW.  Cowpox virus CrmA, Myxoma virus SERP2 and baculovirus P35 are not functionally interchangeable caspase inhibitors in poxvirus infections. J Gen Virol. 2004 May;85(Pt 5):1267-78.  abstract

Rajkumar Nathaniel, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Richard W. Moyer, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1996 - May 2003

Dissertation Title

Role of CrmA in regulating inflammation and apoptosis during cowpox virus infection

Professional Positions

Postdoctoral Associate, University of Illinois

Education

B.S., Bangalore University, India;  M.S., University of Central Florida, Orlando, FL

Publications

Nathaniel R, MacNeill AL, Wang YX, Turner PC, Moyer RW.  Cowpox virus CrmA, Myxoma virus SERP2 and baculovirus P35 are not functionally interchangeable caspase inhibitors in poxvirus infections. J Gen Virol. 2004 May;85(Pt 5):1267-78.  abstract

Macen J, Takahashi A, Moon KB, Nathaniel R, Turner PC, Moyer RW.  Activation of caspases in pig kidney cells infected with wild-type and CrmA/SPI-2 mutants of cowpox and rabbitpox viruses. J Virol. 1998 May;72(5):3524-33.  abstract

Cuong Nguyen, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Ammon B. Peck, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 2001 - August 2006

Dissertation Title

Professional Positions

Education

B.S., Biochemistry, University of Nebraska

Publications

Gao J, Killedar S, Cornelius JG, Nguyen C, Cha S, Peck AB. Sjogren's syndrome in the NOD mouse model is an interleukin-4 time-dependent, antibody isotype-specific autoimmune disease. J Autoimmun. 2006 Mar;26(2):90-103.  abstract

Nguyen C, Cornelius J, Singson E, Killedar S, Cha S, Peck AB. Role of complement and B lymphocytes in Sjogren's syndrome-like autoimmune exocrinopathy of NOD.B10-H2(b) mice. Mol Immunol. 2005 Oct 8; [Epub ahead of print]  abstract

Honors & Awards

Abstracts Presented at Meetings Through Graduate Studies

Jerome E. O'Neil, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

David C. Bloom, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - May 2004

Dissertation Title

An investigation of the Herpes simplex virus type-1 latency associated transcript’s role in the establishment of latency and reactivation

Professional Positions

Law School Student, Albany Law School, Albany, NY

Education

B.S., University of New Hampshire, Durham, NH

Publications

O'Neil JE, Loutsch JM, Aguilar JS, Hill JM, Wagner EK, Bloom DC.  Wide variations in herpes simplex virus type 1 inoculum dose and latency-associated transcript expression phenotype do not alter the establishment of latency in the rabbit eye model. J Virol. 2004 May;78(10):5038-44.  abstract

Joseph Oshier (M.D., Ph.D.)

Advanced Concentration

Immunology & Microbiology

Mentor

Maureen Goodenow, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 2000 - May 2006 (Ph.D. studies)

Dissertation Title

A novel post-entry determinant of macrophage tropism maps to the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein

Professional Positions

Education

B.S., University of Florida

Publications

Chen S, Tuttle DL, Oshier JT, Knot HJ, Streit WJ, Goodenow MM, Harrison JK.  Transforming growth factor-beta1 increases CXCR4 expression, stromal-derived factor-1alpha-stimulated signalling and human immunodeficiency virus-1 entry in human monocyte-derived macrophages. Immunology. 2005 Apr;114(4):565-74.  abstract

Coberley CR, Kohler JJ, Brown JN, Oshier JT, Baker HV, Popp MP, Sleasman JW, Goodenow MM.  Impact on genetic networks in human macrophages by a CCR5 strain of human immunodeficiency virus type 1. J Virol. 2004 Nov;78(21):11477-86.  abstract

Bray CL, Cahill KS, Oshier JT, Peden CS, Theriaque DW, Flotte TR, Stacpoole PW.  Methylphenidate does not improve cognitive function in healthy sleep-deprived young adults. J Investig Med. 2004 Apr;52(3):192-201.  abstract

Nikki R. Rhodin, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

L. Jeannine Brady, Ph.D.

Department

Oral Biology

Enrolled

August 1999 - May 2003

Dissertation Title

Immunomodulation by an anti-Streptococcus mutans monoclonal antibody

Professional Positions

Postdoctoral Associate, Dept. Oral Biology, UF College of Dentistry

Education

B.S., Microbiology & Cell Science, University of Florida

Publications

Rhodin NR, Cutalo JM, Tomer KB, McArthur WP, Brady LJ.  Characterization of the Streptococcus mutans P1 epitope recognized by immunomodulatory monoclonal antibody 6-11A. Infect Immun. 2004 Aug;72(8):4680-8.  abstract

Rhodin NR, Van Tilburg ML, Oli MW, McArthur WP, Brady LJ.  Further characterization of immunomodulation by a monoclonal antibody against Streptococcus mutans antigen P1. Infect Immun. 2004 Jan;72(1):13-21.  abstract

Abstracts Presented at Meetings Through Graduate Studies

Changes in the Serum IgG Response against streptococcus mutans P1 (Antigen I/II) Mediated by an Anti-P1 Monoclonal Antibody Result in Increased Inhibitions of Adherence to Salivary Agglutinin-Coated Hydroxyapatite. M.L.J. van Tilburg, N.R. Rhodin, L.J. Brady. (ASM Abstract Book, 2000, p295, E12) Presented at ASM, 100th General Meeting, May, 2000, Los Angeles, CA

Alterations in Protease Susceptibility of Streptococcus mutans Antigen PI Mediated by Monoclonal Antibody (MA b). N.R. Rhodin, W.P. McArthur, and L.J. Brady. (Volume 81, March 2002, p. A-117, Abstract #0759) Presented at 80th General Session of International Association for Dental Research, March 2002, San Diego CA

Studies of Streptococcus mutans Anti-P1 Monoclonal Antibodies: Evaluation of Intra-Molecular Interactions Necessary for Epitome Formation.
N.R. Rhodin, T.B. Seifert, W.P. McArthur, L.J. Bradley. Presented at 81st General Session of the International Association for Dental Research, June 2003, Gotenborg, Sweden. Abstract #36969.

Honors & Awards

President's Award 2002, Southeastern Branch American Society for Microbiology, Gainesville, FL

Third place, Ph.D./Postdoctoral Research Competition, College of Dentistry Research Day, 2003.

Stephanie L. Rose, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Maureen M. Goodenow, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1998 - August 2002

Dissertation Title

Human immunodeficiency virus type 1 (HIV-1) gag-pro genetic variability is related to therapy response and impacts viral fitness in specific cell types  (full text)

Professional Positions

Postdoctoral Associate, Dept. of Pathology, Immunology & Laboratory Medicine, UF College of Medicine

Education

B.S., Biology, Christopher Newport University

Publications

Goodenow MM, Rose SL, Tuttle DL, Sleasman JW.  HIV-1 fitness and macrophages. J Leukoc Biol. 2003 Nov;74(5):657-66.  abstract

Goodenow MM, Bloom G, Rose SL, Pomeroy SM, O'Brien PO, Perez EE, Sleasman JW, Dunn BM.  Naturally occurring amino acid polymorphisms in human immunodeficiency virus type 1 (HIV-1) Gag p7(NC) and the C-cleavage site impact Gag-Pol processing by HIV-1 protease. Virology. 2002 Jan 5;292(1):137-49.  abstract

Perez EE, Rose SL, Peyser B, Lamers SL, Burkhardt B, Dunn BM, Hutson AD, Sleasman JW, Goodenow MM.  Human immunodeficiency virus type 1 protease genotype predicts immune and viral responses to combination therapy with protease inhibitors (PIs) in PI-naive patients. J Infect Dis. 2001 Feb 15;183(4):579-88.  abstract

Abstracts Presented at Meetings Through Graduate Studies

Rose, S., S. Pomeroy, G. Bloom, J. Sleasman, B. Dunn, and M. Goodenow. Polymorphisms in HIV-1 gag-pol influence both proteolytic processing and replication dynamics. 8th Conference on Retroviruses and Opportunistic Infections, Chicago, IL. February, 2001.

Honors & Awards

Graduate Fellowship for Outstanding Research, UF College of Medicine, 2001

Daniel Saban, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Ammon B. Peck, Ph.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled 

August 2002 - December 2005

Dissertation Title

Immunological characterization of the efferent limb, the afferent limb, and the ocular phase of eye-derived tolerance

Professional Positions

Education

M.S., Molecular Biology, University of Florida;  BS. Biology, George Washington University, Washington, DC

Publications

Sengupta N, Caballero S, Mames RN, Timmers AM, Saban D, Grant MB.  Preventing stem cell incorporation into choroidal neovascularization by targeting homing and attachment factors. Invest Ophthalmol Vis Sci. 2005 Jan;46(1):343-8.  abstract

Abstracts Presented at Meetings

Saban DR, Shwartzkopff J, Peck AB, and Grant MB. "Preferential Production of Distinct IgG Subclasses in Response to Anterior Chamber Immunization with Allosplenocytes. ARVO. 2005, Abstract 5042.

Saban DR, Elder I. Timmers AM, Grant MB, and Peck AB. "The Use of Eye-Derived Tolerance to Inhibit Alloantigen Induced Delayed-Type Hypersensitivity." FOCIS. 12th International Congress of Immunology. 2004, Abstract T33.105.

Haire SE, Elder IA, Saban DR, Janssen JJ, and Timmers AM. "Optimization of Lentiviral Gene Delivery to the RPE in vivo." ARVO. 2003, Abstract 452.

Saban DR, Elder IA, Grant MB, and Timmers AM. "Prolongation of Recombinant Adenoviral Expression through the Modulation of Innate Ocular Immunity." ARVO. 2002, Abstract 2733.

Timmers AM, Nguyen TB, Elder IA, and Saban DR. "Lentiviral Delivery of DH-sCNTF to RPE in P23H Rats Rescues Photoreceptor Cells Morphologically but not Functionally." ARVO. 2002, Abstract 2732.

Timmers AM, Nguyen TB, and Saban DR. "Viral Vectors for Efficient Gene Delivery to RPE Cells." IOVS; S-667 vol. 42-4, March 15, 2001.

Michael Sedlacek, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Clay B. Walker, Ph.D. 

Department

Oral Biology

Enrolled 

August 1998 - December 2005

Dissertation Title

An in vitro biofilm model of subgingival plaque:  Biofilm development and antibiotic susceptibilities

Professional Positions  

Education  

B.S., Microbiology  & Cell Science, University of Florida

Kyle N. Seifert, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

L. Jeannine Brady, Ph.D. 

Department

Oral Biology

Enrolled

August 1998 - May 2004

Dissertation Title

Partial characterization of group B streptococcal surface antigens delta, episilon and glyceraldehyde-3-phosphate dehydrogenase

Professional Positions

Assistant Professor, James Madison University, Department of Biology, Harrisonburg, VA

Education

B.A., Biology, Augustana College, Rock Island, IL

Publications

Seifert KN, McArthur WP, Bleiweis AS, Brady LJ. Characterization of group B streptococcal glyceraldehyde-3-phosphate dehydrogenase: surface localization, enzymatic activity, and protein-protein interactions. Can J Microbiol. 2003 May;49(5):350-6.  abstract

Trevor Seifert, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

L. Jeannine Brady, Ph.D. 

Department

Oral Biology

Enrolled 

August 1998 - May 2005

Dissertation Title

Molecular interactions of the Streptococcus mutans surface protein P1:  Contributions to protein structure, stability, and translocation

Professional Positions

Postdoctoral Fellow, Center for Oral Biology, University of Rochester, May - December 2005

Education

B.S., Biology, Southern Oregon State College

Publications

Seifert TB, Bleiweis AS, Brady LJ.  Contribution of the alanine-rich region of Streptococcus mutans P1 to antigenicity, surface expression, and interaction with the proline-rich repeat domain. Infect Immun. 2004 Aug;72(8):4699-706.  abstract

Gregory Simon, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Barry J. Byrne, M.D., Ph.D. 

Department

Molecular Genetics & Microbiology

Enrolled 

August 2001 - December 2005

Dissertation Title

Novel therapeutic methods for inducing immunomodulation:  Applications to the non-obese diabetic mouse model of Type 1 diabetes

Professional Positions

Education

B.S., IDS, Molecular Genetics (Microbiology & Cell Science), University of Florida

Publications

Walker MC, Mandell TC, Crawford PC, Simon GG, Cahill KS, Fernandes PJ, MacLeod JN, Byrne BJ, Levy JK. Expression of erythropoietin in cats treated with a recombinant adeno-associated viral vector. Am J Vet Res. 2005 Mar;66(3):450-6. abstract

Guthrie SM, Curtis LM, Mames RN, Simon GG, Grant MB, Scott EW.  The nitric oxide pathway modulates hemangioblast activity of adult hematopoietic stem cells. Blood. 2005 Mar 1;105(5):1916-22.  abstract

Jeffrey R. Sirninger, D.V.M., Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Terence R. Flotte, M.D.

Department

Molecular Genetics & Microbiology;  Pediatrics

Enrolled

August 1998 - December 2003

Dissertation Title

Design and testing of gene therapeutics for treating cystic fibrosis  

Professional Positions

Assistant Professor, Dept. Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA

Publications

Platt SR, Graham J, Chrisman CL, Collins K, Chandra S, Sirninger J, Newell SM.  Magnetic resonance imaging and ultrasonography in the diagnosis of a malignant peripheral nerve sheath tumor in a dog. Vet Radiol Ultrasound. 1999 Jul-Aug;40(4):367-71.  abstract

Randell SC, Lewis DD, Newell SM, Uhl EW, Sirninger JR.  What is your diagnosis? An aggressive bony lesion in the left ilium and severe degenerative changes in the right coxal joint and lumbosacral disk space. J Am Vet Med Assoc. 1999 Feb 1;214(3):331-2.  abstract

Angela M. Starks, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Paul A. Gulig, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1997 - May 2003

Dissertation Title

Molecular pathogenesis of Vibrio vulnificus in iron-dextran-treated mice

Professional Positions

Postdoctoral Associate, Dept. Microbiology & Immunology, Emory University, Atlanta, GA

Education

B.S., Biology (Chemistry), Berry College, Rome, GA

Publications

Starks AM, Schoeb TR, Tamplin ML, Parveen S, Doyle TJ, Bomeisl PE, Escudero GM, Gulig PA.  Pathogenesis of infection by clinical and environmental strains of Vibrio vulnificus in iron-dextran-treated mice. Infect Immun. 2000 Oct;68(10):5785-93.  abstract

Jeong KC, Jeong HS, Rhee JH, Lee SE, Chung SS, Starks AM, Escudero GM, Gulig PA, Choi SH. Construction and phenotypic evaluation of a Vibrio vulnificus vvpE mutant for elastolytic protease. Infect Immun. 2000 Sep;68(9):5096-106.  abstract

Abstracts Presented at Meetings Through Graduate Studies

Starks, A.S., K.L. Bourdage, and P.A. Gulig. Use of marker plasmid system for examination of differences in in vivo replication and susceptibility to host defenses of virulent and attenuated strains of Vibrio Vulnificus. Annu Meet. SEB- Am. Soc. Micriobiol. 2002

Starks, A.S., and P.A. Gulig. In vivo selection for isolation of virulence factors of Vibrio vulnificus. Annu Meet. Am. Soc. Microbiol. 2002

Gallman, A.M., T.J. Doyle, S.C. Skinner, P.E. Bomeisl, S. Parveen, M.L. Tamplin, and P.A. Gulig. Pathogenesis of infection by virulent and naturally attenuated strains of Vibrio vulnificus in subcutanouesly inoculated, iron dextran-treated mice. Annu. Meet. Am. Soc. Micriobiol. 1999

Gallman, A.M, D.H. Duckworth, S.C. Skinner, T.J. Doyle, and P.A. Gulig. Both virulent and naturally attenuated V. vulnificus strains are cytotoxic, but non-invasive into, human intestinal epithelial cell lines. Annu. Meet. Am. Soc. Microbiol. 1999

Honors & Awards

Richard and Mary Finkelstein Student Travel Award, ASM 103rd General Meeting, 2003

President's Award, Best Scientific Paper, Annual Meeting of the SE Branch of the American Society for Microbiology, 2002

Medical Guild Grad. Student Research Competition, UF College of Medicine, 2002, 1st Place Immunology & Microbiology, 1st Place Overall

Predoctoral Fellowship, Florida Affiliate of the American Heart Association, 2000-2002

Graduate Fellowship funded by NIH Infectious Disease Training Grant

Robert K. Tran, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

David C. Bloom, Ph.D.

Department

Molecular Genetics & Microbiology

Enrolled

August 1999 - December 2002

Dissertation Title

Investigation of HSV gene regulation by methylation profiling and promoter replacement assay

Professional Positions

Postdoctoral Associate, Fred Hutchinson Cancer Research Center, Seattle, WA

Education

B.S., University of California at Los Angeles, Los Angeles, CA

Publications

Kubat NJ, Tran RK, McAnany P, Bloom DC.  Specific histone tail modification and not DNA methylation is a determinant of herpes simplex virus type 1 latent gene expression. J Virol. 2004 Feb;78(3):1139-49.  abstract

Bhattacharjee PS, Tran RK, Myles ME, Maruyama K, Mallakin A, Bloom DC, Hill JM.  Overlapping subdeletions within a 348-bp in the 5' exon of the LAT region that facilitates epinephrine-induced reactivation of HSV-1 in the rabbit ocular model do not further define a functional element. Virology. 2003 Jul 20;312(1):151-8.  abstract

Vallur AC, Feller JA, Abner CW, Tran RK, Bloom LB.  Effects of hydrogen bonding within a damaged base pair on the activity of wild type and DNA-intercalating mutants of human alkyladenine DNA glycosylase. J Biol Chem. 2002 Aug 30;277(35):31673-8.  abstract

Tran RK, Lieu PT, Aguilar S, Wagner EK, Bloom DC.  Altering the expression kinetics of VP5 results in altered virulence and pathogenesis of herpes simplex virus type 1 in mice. J Virol. 2002 Mar;76(5):2199-205.  abstract

Marquart ME, Zheng X, Tran RK, Thompson HW, Bloom DC, Hill JM.  A cAMP response element within the latency-associated transcript promoter of HSV-1 facilitates induced ocular reactivation in a mouse hyperthermia model. Virology. 2001 May 25;284(1):62-9.  abstract

Tabbaa S, Goulah C, Tran RK, Lis A, Korody R, Stachowski B, Horowitz JM, Torres G, Stachowiak EK, Bloom DC, Stachowiak MK.  Gene transfer into the central nervous system using herpes simplex virus-1 vectors. Folia Morphol (Warsz). 2000;59(4):221-32.  abstract

Bloom DC, Stevens JG, Hill JM, Tran RK.  Mutagenesis of a cAMP response element within the latency-associated transcript promoter of HSV-1 reduces adrenergic reactivation. Virology. 1997 Sep 15;236(1):202-7.  abstract

Donna S. Whittaker, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Michael J. Clare-Salzler, M.D.

Department

Pathology, Immunology & Laboratory Medicine

Enrolled

August 1996 - August 2000

Dissertation Title

The role of prostaglandins in the maturation and function of human monocyte derived dendritic cells

Professional Positions

Chief, Blood Bank, Brooke Army Medical Center, Ft. Sam Houston, TX

Education

B.S., University of Georgia, Athens, GA

M.H.S., Duke University, Durham, NC

Publications

Litherland SA, She JX, Schatz D, Fuller K, Hutson AD, Peng RH, Li Y, Grebe KM, Whittaker DS, Bahjat K, Hopkins D, Fang Q, Spies PD, North K, Wasserfall C, Cook R, Dennis MA, Crockett S, Sleasman J, Kocher J, Muir A, Silverstein J, Atkinson M, Clare-Salzler MJ.  Aberrant monocyte prostaglandin synthase 2 (PGS2) expression in type 1 diabetes before and after disease onset. Pediatr Diabetes. 2003 Mar;4(1):10-8.  abstract

Whittaker DS, Bahjat KS, Moldawer LL, Clare-Salzler MJ.  Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production. J Immunol. 2000 Oct 15;165(8):4298-304.  abstract

Litherland SA, Xie XT, Hutson AD, Wasserfall C, Whittaker DS, She JX, Hofig A, Dennis MA, Fuller K, Cook R, Schatz D, Moldawer LL, Clare-Salzler MJ.  Aberrant prostaglandin synthase 2 expression defines an antigen-presenting cell defect for insulin-dependent diabetes mellitus. J Clin Invest. 1999 Aug;104(4):515-23.  abstract

Honors & Awards

Graduate Student of the Year, University of Florida, 1999

Lihui Yuan, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Ann Progulske-Fox, Ph.D.

Department

Oral Biology

Enrolled 

August 1999 - May 2005

Dissertation Title

Quorum sensing regulated gene expression in Porphyromonas gingivalis

Professional Positions

Postdoctoral Fellow, Department of Oral Biology, UF College of Dentistry

Education

B.S., M.S., Agronomy, Beijing University

Publications

Yuan L, Hillman JD, Progulske-Fox A.  Microarray analysis of quorum-sensing-regulated genes in Porphyromonas gingivalis. Infect Immun. 2005 Jul;73(7):4146-54.  abstract

Lin Zeng, Ph.D.

Advanced Concentration

Immunology & Microbiology

Mentor

Shouguang Jin, Ph.D. 

Department

Molecular Genetics & Microbiology

Enrolled

January 2000 - May 2004

Dissertation Title

Pseudomonas aeruginosa pathogenicity and antibiotic resistance

Professional Positions

Postdoctoral Associate, Dept. Molecular Genetics & Microbiology, UF College of Medicine

Education

B.S., M.S., NMicrobiology, Nankai University, P.R. China

Publications

Zeng L, Jin S.  aph(3')-IIb, a gene encoding an aminoglycoside-modifying enzyme, is under the positive control of surrogate regulator HpaA. Antimicrob Agents Chemother. 2003 Dec;47(12):3867-76.  abstract

Wang Y, Ha U, Zeng L, Jin S.  Regulation of membrane permeability by a two-component regulatory system in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2003 Jan;47(1):95-101.  abstract

Kaufman MR, Jia J, Zeng L, Ha U, Chow M, Jin S.  Pseudomonas aeruginosa mediated apoptosis requires the ADP-ribosylating activity of exoS. Microbiology. 2000 Oct;146 ( Pt 10):2531-41.  abstract